tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2012 Apr 4. [Epub ahead of print]
Evaluation of influenza A/H3N2 and B vaccines on the basis of cross-reactivity of post-vaccination human serum antibodies against influenza A/H3N2 and B viruses isolated in MDCK cells and embryonated hen eggs.
Kishida N, Fujisaki S, Yokoyama M, Sato H, Saito R, Ikematsu H, Xu H, Takashita E, Tashiro M, Takao S, Yano T, Suga T, Kawakami C, Yamamoto M, Kajiyama K, Saito H, Shimada S, Watanabe S, Aoki S, Taira K, Kon M, Lin JH, Odagiri T.
Source
Laboratory of Influenza Virus Surveillance, Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
Abstract
The influenza vaccines of A/H3N2 for the 2010/11 season and of B for the 2009/10 and 2010/11 seasons in Japan are a high-growth reassortant A/Victoria/210/2009 (X-187) strain and an egg-adapted B/Brisbane/60/2008 (Victoria-lineage) strain, respectively. Hemagglutination-inhibition (HI) tests with post-infection ferret antisera indicated that the antisera raised against the X-187 and egg-adapted B/Brisbane/60/2008 vaccine production strains poorly inhibited recent epidemic isolates of MDCK-grown A/H3N2 and B/Victoria-lineage viruses, respectively. The low reactivity of the ferret antisera would be attributable to changes in the hemagglutinin (HA) protein of production strains during egg-adaptation. To evaluate the efficacy of A/H3N2 and B vaccines, the cross-reactivities of post-vaccination human serum antibodies against A/H3N2 and B/Victoria-lineage epidemic isolates were assessed by a comparison of the geometric mean titers (GMTs) of HI and neutralization (NT) tests. Serum antibodies elicited by the X-187 vaccine had low cross-reactivity to both MDCK- and egg-grown A/H3N2 isolates by HI test and narrow cross-reactivity by NT test in all age groups. On the other hand, the GMTs to B viruses detected by HI test were below the marginal level, so the cross-reactivity was assessed by NT test. The serum neutralizing antibodies elicited by B/Brisbane/60/2008 vaccine reacted well with egg-grown B viruses, but exhibited remarkably low reactivity to MDCK-grown B viruses. The results of these human serological studies suggest that the influenza A/H3N2 vaccine for the 2010/11 season and B vaccine for the 2009/10 and 2010/11 seasons would possess insufficient efficacy and less efficacy, respectively.
PMID:
22492743
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22492743
Evaluation of influenza A/H3N2 and B vaccines on the basis of cross-reactivity of post-vaccination human serum antibodies against influenza A/H3N2 and B viruses isolated in MDCK cells and embryonated hen eggs.
Kishida N, Fujisaki S, Yokoyama M, Sato H, Saito R, Ikematsu H, Xu H, Takashita E, Tashiro M, Takao S, Yano T, Suga T, Kawakami C, Yamamoto M, Kajiyama K, Saito H, Shimada S, Watanabe S, Aoki S, Taira K, Kon M, Lin JH, Odagiri T.
Source
Laboratory of Influenza Virus Surveillance, Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
Abstract
The influenza vaccines of A/H3N2 for the 2010/11 season and of B for the 2009/10 and 2010/11 seasons in Japan are a high-growth reassortant A/Victoria/210/2009 (X-187) strain and an egg-adapted B/Brisbane/60/2008 (Victoria-lineage) strain, respectively. Hemagglutination-inhibition (HI) tests with post-infection ferret antisera indicated that the antisera raised against the X-187 and egg-adapted B/Brisbane/60/2008 vaccine production strains poorly inhibited recent epidemic isolates of MDCK-grown A/H3N2 and B/Victoria-lineage viruses, respectively. The low reactivity of the ferret antisera would be attributable to changes in the hemagglutinin (HA) protein of production strains during egg-adaptation. To evaluate the efficacy of A/H3N2 and B vaccines, the cross-reactivities of post-vaccination human serum antibodies against A/H3N2 and B/Victoria-lineage epidemic isolates were assessed by a comparison of the geometric mean titers (GMTs) of HI and neutralization (NT) tests. Serum antibodies elicited by the X-187 vaccine had low cross-reactivity to both MDCK- and egg-grown A/H3N2 isolates by HI test and narrow cross-reactivity by NT test in all age groups. On the other hand, the GMTs to B viruses detected by HI test were below the marginal level, so the cross-reactivity was assessed by NT test. The serum neutralizing antibodies elicited by B/Brisbane/60/2008 vaccine reacted well with egg-grown B viruses, but exhibited remarkably low reactivity to MDCK-grown B viruses. The results of these human serological studies suggest that the influenza A/H3N2 vaccine for the 2010/11 season and B vaccine for the 2009/10 and 2010/11 seasons would possess insufficient efficacy and less efficacy, respectively.
PMID:
22492743
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22492743