tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2019 Sep 21. doi: 10.1111/irv.12678. [Epub ahead of print]
[h=1]Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus-infected ferrets.[/h] Bekking C[SUP]1,[/SUP][SUP]2[/SUP], Yip L[SUP]2[/SUP], Groulx N[SUP]2[/SUP], Doggett N[SUP]1,[/SUP][SUP]2[/SUP], Finn M[SUP]1,[/SUP][SUP]2[/SUP], Mubareka S[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h] 1 Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. 2 Biological Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally-infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will provide insight for device use.
[h=4]OBJECTIVES:[/h] The primary objective of this study was to assess and compare bioaerosol sampling devices using a) an in vitro, environmentally-controlled artificial bioaerosol system at a range of different RH conditions and b) an in vivo bioaerosol system of influenza virus-infected ferrets under controlled environmental conditions. Secondarily, we also sought to examine the impact of NSAIDs on bioaerosol emission in influenza virus-infected ferrets to address its potential as a determinant of bioaerosol emission.
[h=4]METHODS:[/h] We examined the performance of low and moderate volume bioaerosol samplers for the collection of viral RNA and infectious influenza virus in vitroand in vivo using artificial bioaerosols and the ferret model of influenza virus infection. The following samplers were tested: the polytetrafluoroethylene filter (PTFE filter), the 2-stage National Institute of Occupational Safety and Health cyclone sampler (NIOSH cyclone sampler) and the 6-stage viable Andersen impactor (Andersen impactor).
[h=4]RESULTS:[/h] The PTFE filter and NIOSH cyclone sampler collected similar amounts of viral RNA and infectious virus from artificially-generated aerosols under a range of relative humidities (RH). Using the ferret model, the PTFE filter, NIOSH cyclone sampler and the Andersen impactor collected up to 3.66 log[SUB]10[/SUB] copies of RNA/L air, 3.84 log[SUB]10[/SUB] copies of RNA/L air and 6.09 log[SUB]10[/SUB] copies of RNA/L air respectively at peak recovery. Infectious virus was recovered from the PTFE filter and NIOSH cyclone samplers on the peak day of viral RNA recovery.
[h=4]CONCLUSION:[/h] The PTFE filter and NIOSH cyclone sampler are useful for influenza virus RNA and infectious virus collection and may be considered for clinical and environmental settings.
? 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] bioaerosol samplers; bioaerosols; ferret model; influenza virus
PMID: 31541519 DOI: 10.1111/irv.12678
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[h=1]Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus-infected ferrets.[/h] Bekking C[SUP]1,[/SUP][SUP]2[/SUP], Yip L[SUP]2[/SUP], Groulx N[SUP]2[/SUP], Doggett N[SUP]1,[/SUP][SUP]2[/SUP], Finn M[SUP]1,[/SUP][SUP]2[/SUP], Mubareka S[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h] 1 Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. 2 Biological Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally-infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will provide insight for device use.
[h=4]OBJECTIVES:[/h] The primary objective of this study was to assess and compare bioaerosol sampling devices using a) an in vitro, environmentally-controlled artificial bioaerosol system at a range of different RH conditions and b) an in vivo bioaerosol system of influenza virus-infected ferrets under controlled environmental conditions. Secondarily, we also sought to examine the impact of NSAIDs on bioaerosol emission in influenza virus-infected ferrets to address its potential as a determinant of bioaerosol emission.
[h=4]METHODS:[/h] We examined the performance of low and moderate volume bioaerosol samplers for the collection of viral RNA and infectious influenza virus in vitroand in vivo using artificial bioaerosols and the ferret model of influenza virus infection. The following samplers were tested: the polytetrafluoroethylene filter (PTFE filter), the 2-stage National Institute of Occupational Safety and Health cyclone sampler (NIOSH cyclone sampler) and the 6-stage viable Andersen impactor (Andersen impactor).
[h=4]RESULTS:[/h] The PTFE filter and NIOSH cyclone sampler collected similar amounts of viral RNA and infectious virus from artificially-generated aerosols under a range of relative humidities (RH). Using the ferret model, the PTFE filter, NIOSH cyclone sampler and the Andersen impactor collected up to 3.66 log[SUB]10[/SUB] copies of RNA/L air, 3.84 log[SUB]10[/SUB] copies of RNA/L air and 6.09 log[SUB]10[/SUB] copies of RNA/L air respectively at peak recovery. Infectious virus was recovered from the PTFE filter and NIOSH cyclone samplers on the peak day of viral RNA recovery.
[h=4]CONCLUSION:[/h] The PTFE filter and NIOSH cyclone sampler are useful for influenza virus RNA and infectious virus collection and may be considered for clinical and environmental settings.
? 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] bioaerosol samplers; bioaerosols; ferret model; influenza virus
PMID: 31541519 DOI: 10.1111/irv.12678
Free full text