tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2018 Mar 12. pii: S0732-8893(18)30085-3. doi: 10.1016/j.diagmicrobio.2018.03.007. [Epub ahead of print]
[h=1]Evaluation of a two-stage testing algorithm for the diagnosis of respiratory viral infections.[/h] Gardiner BJ[SUP]1[/SUP], Parker CEV[SUP]2[/SUP], Rabson AR[SUP]3[/SUP], Snydman DR[SUP]4[/SUP], Doron S[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] New on-demand multiplex molecular respiratory viral diagnostics offer superior performance although can be expensive and some platforms cannot process multiple specimens simultaneously. We performed a retrospective study reviewing results of patients tested for respiratory viruses following introduction of a two-stage testing algorithm incorporating an initial screen with Sofia? immunoassay then secondary Biofire Filmarray?, and compared to a period when only Filmarray? was used. Of 2976 testing episodes, 1814 underwent initial Sofia? then follow-up FilmArray?. A diagnosis of influenza was made by Sofia? in 282 patients, and by FilmArray? in an additional 163 (median time to result 1.12hours versus 3.46hours, P<0.001). Significantly more patients received their diagnosis within 90minutes in winter despite testing more samples (11.1% versus 3.4%, P<0.001), and approximately $36,000 was saved. An algorithmic approach to respiratory viral diagnosis can combine the advantages of accuracy and speed and be cost saving.
[h=4]KEYWORDS:[/h] Diagnosis; Influenza; PCR; Respiratory viral infection
PMID: 29636246 DOI: 10.1016/j.diagmicrobio.2018.03.007
[h=1]Evaluation of a two-stage testing algorithm for the diagnosis of respiratory viral infections.[/h] Gardiner BJ[SUP]1[/SUP], Parker CEV[SUP]2[/SUP], Rabson AR[SUP]3[/SUP], Snydman DR[SUP]4[/SUP], Doron S[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] New on-demand multiplex molecular respiratory viral diagnostics offer superior performance although can be expensive and some platforms cannot process multiple specimens simultaneously. We performed a retrospective study reviewing results of patients tested for respiratory viruses following introduction of a two-stage testing algorithm incorporating an initial screen with Sofia? immunoassay then secondary Biofire Filmarray?, and compared to a period when only Filmarray? was used. Of 2976 testing episodes, 1814 underwent initial Sofia? then follow-up FilmArray?. A diagnosis of influenza was made by Sofia? in 282 patients, and by FilmArray? in an additional 163 (median time to result 1.12hours versus 3.46hours, P<0.001). Significantly more patients received their diagnosis within 90minutes in winter despite testing more samples (11.1% versus 3.4%, P<0.001), and approximately $36,000 was saved. An algorithmic approach to respiratory viral diagnosis can combine the advantages of accuracy and speed and be cost saving.
[h=4]KEYWORDS:[/h] Diagnosis; Influenza; PCR; Respiratory viral infection
PMID: 29636246 DOI: 10.1016/j.diagmicrobio.2018.03.007