tetano
Editor, Senior Moderator
J Virol Methods. 2019 Jan 8. pii: S0166-0934(18)30328-8. doi: 10.1016/j.jviromet.2019.01.004. [Epub ahead of print]
[h=1]Evaluation of a rapid isothermal nucleic acid amplification kit, Alere? i Influenza A&B, for the detection of avian influenza viruses.[/h] Bazarragchaa E[SUP]1[/SUP], Okamatsu M[SUP]2[/SUP], Ulaankhuu A[SUP]3[/SUP], Twabela AT[SUP]2[/SUP], Matsuno K[SUP]4[/SUP], Kida H[SUP]5[/SUP], Sakoda Y[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid and accurate diagnosis of influenza virus infection is essential for quick responses for both human and animal health. The Alere? i Influenza A&B is a novel isothermal nucleic acid amplification kit that can detect and differentiate between influenza A and B viruses in human specimens in approximately 15 minutes. In the present study, the performance of the Alere? i Influenza A&B kit was evaluated for its ability to detect avian influenza virus in chickens. The kit was able to detect representative avian influenza virus strains (hemagglutinin subtypes H1-H16, including the recently isolated H5 and H7 highly pathogenic avian influenza viruses), and the detection limit of the kit for these viruses varied between 10[SUP]-1.4[/SUP]-10[SUP]2.1[/SUP] 50% egg-infective dose per test, which is higher than the analytical sensitivity of the antigen detection immunochromatography kit ESPLINE[SUP]?[/SUP] A INFLUENZA. In experimentally infected chickens inoculated with a highly pathogenic avian influenza virus strain A/chicken/Hokkaido/002/2016 (H5N6), viral RNA was detected in the tracheal and cloacal swabs. These results indicate that this kit has the potential to be used as a rapid screening test of influenza A virus infection in chickens.
Copyright ? 2019. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] amplification; avian influenza; chicken; nucleic acid; rapid detection
PMID: 30633948 DOI: 10.1016/j.jviromet.2019.01.004
[h=1]Evaluation of a rapid isothermal nucleic acid amplification kit, Alere? i Influenza A&B, for the detection of avian influenza viruses.[/h] Bazarragchaa E[SUP]1[/SUP], Okamatsu M[SUP]2[/SUP], Ulaankhuu A[SUP]3[/SUP], Twabela AT[SUP]2[/SUP], Matsuno K[SUP]4[/SUP], Kida H[SUP]5[/SUP], Sakoda Y[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid and accurate diagnosis of influenza virus infection is essential for quick responses for both human and animal health. The Alere? i Influenza A&B is a novel isothermal nucleic acid amplification kit that can detect and differentiate between influenza A and B viruses in human specimens in approximately 15 minutes. In the present study, the performance of the Alere? i Influenza A&B kit was evaluated for its ability to detect avian influenza virus in chickens. The kit was able to detect representative avian influenza virus strains (hemagglutinin subtypes H1-H16, including the recently isolated H5 and H7 highly pathogenic avian influenza viruses), and the detection limit of the kit for these viruses varied between 10[SUP]-1.4[/SUP]-10[SUP]2.1[/SUP] 50% egg-infective dose per test, which is higher than the analytical sensitivity of the antigen detection immunochromatography kit ESPLINE[SUP]?[/SUP] A INFLUENZA. In experimentally infected chickens inoculated with a highly pathogenic avian influenza virus strain A/chicken/Hokkaido/002/2016 (H5N6), viral RNA was detected in the tracheal and cloacal swabs. These results indicate that this kit has the potential to be used as a rapid screening test of influenza A virus infection in chickens.
Copyright ? 2019. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] amplification; avian influenza; chicken; nucleic acid; rapid detection
PMID: 30633948 DOI: 10.1016/j.jviromet.2019.01.004