tetano
Editor, Senior Moderator
Eur J Public Health
. 2025 Jan 1;35(Supplement_1):i55-i59.
doi: 10.1093/eurpub/ckae106. Molecular diagnosis and phylogenetic analysis of a Middle East respiratory syndrome coronavirus human case in Jordan
Ehab A Abu-Basha[SUP] 1 [/SUP], Zuhair B Ismail[SUP] 1 [/SUP], Mohammad H Alboom[SUP] 1 [/SUP], Ayesha Alkofahi[SUP] 1 [/SUP], Basil H Amarneh[SUP] 1 [/SUP], Omar Al-Omari[SUP] 1 [/SUP], Alaa Fahmawi[SUP] 1 [/SUP], Abdullah Alshammari[SUP] 1 [/SUP], Mais Lakaideh[SUP] 1 [/SUP], Shereen Shaban[SUP] 1 [/SUP], Bilal Al-Omari[SUP] 1 [/SUP], Hani Talafha[SUP] 1 [/SUP], Zaidoun Hijazeen[SUP] 1 [/SUP], Yasmin Daradkeh[SUP] 1 [/SUP], Rabeh El-Shesheny[SUP] 2 [/SUP], Ghazi Kayali[SUP] 3 [/SUP], Whitney Bagge[SUP] 4 [/SUP], William B Karesh[SUP] 4 [/SUP]
Affiliations
Middle East respiratory syndrome coronavirus (MERS-CoV) is an important zoonotic pathogen. The aim of this paper is to report one polymerase chain reaction (PCR)-positive case of MERS-CoV in a 27-year-old man who was involved in a nationwide longitudinal surveillance study of certain zoonotic diseases in Jordan including MERS-CoV. Whole-blood and nasal swab samples were collected from the man and five camels in the vicinity of his living area. The samples were subjected to enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV-specific antibodies and MERS-CoV genetic material, respectively. Genomic sequencing and phylogenetic analysis were also performed to detect similarities with known strains of the virus in the region. In January 2021, an ongoing surveillance study detected a MERS-CoV-positive nasal swab sample from an asymptomatic male and camels using RT-PCR. Phylogenetically, the MERS-CoV isolated in this case belonged to clade B and is clustered with other strains originating in the Arabian Peninsula. The case report represents the first PCR-positive case of MERS-CoV in an asymptomatic individual in Jordan, indicating active circulation of the virus within the population.
. 2025 Jan 1;35(Supplement_1):i55-i59.
doi: 10.1093/eurpub/ckae106. Molecular diagnosis and phylogenetic analysis of a Middle East respiratory syndrome coronavirus human case in Jordan
Ehab A Abu-Basha[SUP] 1 [/SUP], Zuhair B Ismail[SUP] 1 [/SUP], Mohammad H Alboom[SUP] 1 [/SUP], Ayesha Alkofahi[SUP] 1 [/SUP], Basil H Amarneh[SUP] 1 [/SUP], Omar Al-Omari[SUP] 1 [/SUP], Alaa Fahmawi[SUP] 1 [/SUP], Abdullah Alshammari[SUP] 1 [/SUP], Mais Lakaideh[SUP] 1 [/SUP], Shereen Shaban[SUP] 1 [/SUP], Bilal Al-Omari[SUP] 1 [/SUP], Hani Talafha[SUP] 1 [/SUP], Zaidoun Hijazeen[SUP] 1 [/SUP], Yasmin Daradkeh[SUP] 1 [/SUP], Rabeh El-Shesheny[SUP] 2 [/SUP], Ghazi Kayali[SUP] 3 [/SUP], Whitney Bagge[SUP] 4 [/SUP], William B Karesh[SUP] 4 [/SUP]
Affiliations
- PMID: 39801333
- DOI: 10.1093/eurpub/ckae106
Middle East respiratory syndrome coronavirus (MERS-CoV) is an important zoonotic pathogen. The aim of this paper is to report one polymerase chain reaction (PCR)-positive case of MERS-CoV in a 27-year-old man who was involved in a nationwide longitudinal surveillance study of certain zoonotic diseases in Jordan including MERS-CoV. Whole-blood and nasal swab samples were collected from the man and five camels in the vicinity of his living area. The samples were subjected to enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV-specific antibodies and MERS-CoV genetic material, respectively. Genomic sequencing and phylogenetic analysis were also performed to detect similarities with known strains of the virus in the region. In January 2021, an ongoing surveillance study detected a MERS-CoV-positive nasal swab sample from an asymptomatic male and camels using RT-PCR. Phylogenetically, the MERS-CoV isolated in this case belonged to clade B and is clustered with other strains originating in the Arabian Peninsula. The case report represents the first PCR-positive case of MERS-CoV in an asymptomatic individual in Jordan, indicating active circulation of the virus within the population.