tetano
Editor, Senior Moderator
Eur J Med Res
. 2021 Dec 17;26(1):147.
doi: 10.1186/s40001-021-00608-5.
Establishment of a quantitative RT-PCR detection of SARS-CoV-2 virus
Yushen Jiang[SUP] 1 [/SUP], Shanming Zhang[SUP] 2 [/SUP], Hong Qin[SUP] 2 [/SUP], Shuai Meng[SUP] 3 [/SUP], Xuyi Deng[SUP] 4 [/SUP], He Lin[SUP] 5 [/SUP], Xiaoliang Xin[SUP] 3 [/SUP], Yuxin Liang[SUP] 3 [/SUP], Bowen Chen[SUP] 3 [/SUP], Yan Cui[SUP] 3 [/SUP], YiHeng Su[SUP] 3 [/SUP], Pei Liang[SUP] 3 [/SUP], GuangZhi Zhou[SUP] 6 [/SUP], Hongbo Hu[SUP] 6 [/SUP]
Affiliations
Abstract
Background: The outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study.
Methods: RT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated.
Results: The performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/μL and lower limit of quantification (LLOQ) as 10E2 copies/μL.
Conclusion: A quantitative detection of the COVID-19 virus based on RT-PCR was established.
Keywords: COVID-19; Quantitative; RNA; RT-PCR; SARS-CoV-2 virus.
. 2021 Dec 17;26(1):147.
doi: 10.1186/s40001-021-00608-5.
Establishment of a quantitative RT-PCR detection of SARS-CoV-2 virus
Yushen Jiang[SUP] 1 [/SUP], Shanming Zhang[SUP] 2 [/SUP], Hong Qin[SUP] 2 [/SUP], Shuai Meng[SUP] 3 [/SUP], Xuyi Deng[SUP] 4 [/SUP], He Lin[SUP] 5 [/SUP], Xiaoliang Xin[SUP] 3 [/SUP], Yuxin Liang[SUP] 3 [/SUP], Bowen Chen[SUP] 3 [/SUP], Yan Cui[SUP] 3 [/SUP], YiHeng Su[SUP] 3 [/SUP], Pei Liang[SUP] 3 [/SUP], GuangZhi Zhou[SUP] 6 [/SUP], Hongbo Hu[SUP] 6 [/SUP]
Affiliations
- PMID: 34920757
- DOI: 10.1186/s40001-021-00608-5
Abstract
Background: The outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study.
Methods: RT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated.
Results: The performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/μL and lower limit of quantification (LLOQ) as 10E2 copies/μL.
Conclusion: A quantitative detection of the COVID-19 virus based on RT-PCR was established.
Keywords: COVID-19; Quantitative; RNA; RT-PCR; SARS-CoV-2 virus.