tetano
Editor, Senior Moderator
J Gen Virol. 2013 Mar 13. [Epub ahead of print]
Establishment of Vero cell RNA polymerase I-driven reverse genetics for influenza A virus and its application for pandemic (H1N1) 2009 influenza virus vaccine production.
Song MS, Baek YH, Pascua PN, Kwon HI, Park SJ, Kim EH, Lim GJ, Choi YK.
Source
Chungbuk National University.
Abstract
Summary The constant threat of newly emerging influenza viruses with pandemic potential requires the need for prompt vaccine production. Here we utilized the Vero cell polymerase I (PolI) promoter rather than the commonly used human PolI promoter in an established reverse-genetics system for rescuing viable influenza viruses in Vero cells, an approved cell line for human vaccine production. The Vero PolI promoter was more efficient in Vero cells and demonstrated enhanced transcription level and virus rescue rates commensurate to that of the human RNA PolI promoter in 293T cells. Apparently, these result were associated with more efficient generation of A( H1N1)pdm09- and H5N1-derived vaccine seed viruses in Vero cells while rescue rates in 293T cells were comparable. Our study provides an alternative means for improving vaccine preparation by using a novel reverse genetics system for generating influenza A viruses. Keywords: Pandemic influenza, Vaccine, Reverse genetics system, Vero cell, PolI promoter.
PMID:
23486669
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23486669
Establishment of Vero cell RNA polymerase I-driven reverse genetics for influenza A virus and its application for pandemic (H1N1) 2009 influenza virus vaccine production.
Song MS, Baek YH, Pascua PN, Kwon HI, Park SJ, Kim EH, Lim GJ, Choi YK.
Source
Chungbuk National University.
Abstract
Summary The constant threat of newly emerging influenza viruses with pandemic potential requires the need for prompt vaccine production. Here we utilized the Vero cell polymerase I (PolI) promoter rather than the commonly used human PolI promoter in an established reverse-genetics system for rescuing viable influenza viruses in Vero cells, an approved cell line for human vaccine production. The Vero PolI promoter was more efficient in Vero cells and demonstrated enhanced transcription level and virus rescue rates commensurate to that of the human RNA PolI promoter in 293T cells. Apparently, these result were associated with more efficient generation of A( H1N1)pdm09- and H5N1-derived vaccine seed viruses in Vero cells while rescue rates in 293T cells were comparable. Our study provides an alternative means for improving vaccine preparation by using a novel reverse genetics system for generating influenza A viruses. Keywords: Pandemic influenza, Vaccine, Reverse genetics system, Vero cell, PolI promoter.
PMID:
23486669
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23486669