tetano
Editor, Senior Moderator
Arch Virol. 2018 Feb 21. doi: 10.1007/s00705-018-3773-2. [Epub ahead of print]
[h=1]Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses.[/h] Yang F[SUP]1[/SUP], Wu H[SUP]2[/SUP], Liu F[SUP]1[/SUP], Lu X[SUP]1[/SUP], Peng X[SUP]1[/SUP], Wu N[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The H6 subtype avian influenza viruses (AIVs) possess the capacity for zoonotic transmission from avian species to humans. Establishment of a specific, rapid and sensitive method to screen H6 AIVs is necessary. Based on the conserved domain of the matrix and H6 AIV hemagglutinin genes, two TaqMan minor-groove-binder probes and multiplex real-time RT-PCR primers were designed in this study. The multiplex real-time RT-PCR assay developed in this study had high specificity and repeatability and a detection limit of 30 copies per reaction. This rapid diagnostic method will be useful for clinical detection and surveillance of H6 AIVs in China.
[h=4]KEYWORDS:[/h] Avian influenza virus; Detection; H6 subtype; Rapid; Sensitivity
PMID: 29468361 DOI: 10.1007/s00705-018-3773-2
[h=1]Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses.[/h] Yang F[SUP]1[/SUP], Wu H[SUP]2[/SUP], Liu F[SUP]1[/SUP], Lu X[SUP]1[/SUP], Peng X[SUP]1[/SUP], Wu N[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The H6 subtype avian influenza viruses (AIVs) possess the capacity for zoonotic transmission from avian species to humans. Establishment of a specific, rapid and sensitive method to screen H6 AIVs is necessary. Based on the conserved domain of the matrix and H6 AIV hemagglutinin genes, two TaqMan minor-groove-binder probes and multiplex real-time RT-PCR primers were designed in this study. The multiplex real-time RT-PCR assay developed in this study had high specificity and repeatability and a detection limit of 30 copies per reaction. This rapid diagnostic method will be useful for clinical detection and surveillance of H6 AIVs in China.
[h=4]KEYWORDS:[/h] Avian influenza virus; Detection; H6 subtype; Rapid; Sensitivity
PMID: 29468361 DOI: 10.1007/s00705-018-3773-2