tetano
Editor, Senior Moderator
Vet Microbiol. 2010 May 31. [Epub ahead of print]
Erythrocyte binding preference of 16 subtypes of low pathogenic avian influenza and 2009 pandemic influenza A (H1N1) viruses.
Wiriyarat W, Lerdsamran H, Pooruk P, Webster RG, Louisirirotchanakul S, Ratanakorn P, Chaichoune K, Nateerom K, Puthavathana P.
Faculty of Veterinary Science, Mahidol University, Nakhonpathom 73170, Thailand.
Abstract
All 16 subtypes of avian influenza viruses of low pathogenicity (LPAIV) as well as their hemagglutinin (H) antigens, and four 2009 pandemic influenza A (H1N1) virus isolates were assayed for hemagglutinating activity against 5 erythrocyte species: goose, guinea pig, human group O, chicken and horse. Of all viruses and antigens assayed, the highest hemagglutination (HA) titers were obtained with goose and guinea pig erythrocytes. Hemagglutinating activity of replicating LPAIV and LPAIV antigens decreased, in order, with chicken and human group O; meanwhile, horse erythrocytes yielded lowest or no HA titer. Moreover, the 2009 pandemic viruses did not agglutinate both horse and chicken erythrocytes. Our study concluded that goose and guinea pig erythrocytes are the best in HA assay for all subtypes of influenza viruses. Copyright ? 2010. Published by Elsevier B.V.
PMID: 20579820 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20579820
Erythrocyte binding preference of 16 subtypes of low pathogenic avian influenza and 2009 pandemic influenza A (H1N1) viruses.
Wiriyarat W, Lerdsamran H, Pooruk P, Webster RG, Louisirirotchanakul S, Ratanakorn P, Chaichoune K, Nateerom K, Puthavathana P.
Faculty of Veterinary Science, Mahidol University, Nakhonpathom 73170, Thailand.
Abstract
All 16 subtypes of avian influenza viruses of low pathogenicity (LPAIV) as well as their hemagglutinin (H) antigens, and four 2009 pandemic influenza A (H1N1) virus isolates were assayed for hemagglutinating activity against 5 erythrocyte species: goose, guinea pig, human group O, chicken and horse. Of all viruses and antigens assayed, the highest hemagglutination (HA) titers were obtained with goose and guinea pig erythrocytes. Hemagglutinating activity of replicating LPAIV and LPAIV antigens decreased, in order, with chicken and human group O; meanwhile, horse erythrocytes yielded lowest or no HA titer. Moreover, the 2009 pandemic viruses did not agglutinate both horse and chicken erythrocytes. Our study concluded that goose and guinea pig erythrocytes are the best in HA assay for all subtypes of influenza viruses. Copyright ? 2010. Published by Elsevier B.V.
PMID: 20579820 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20579820