tetano
Editor, Senior Moderator
Methods Mol Biol. 2014;1161:403-10. doi: 10.1007/978-1-4939-0758-8_35.
Equine influenza culture methods.
Chambers TM1, Reedy SE.
Author information
Abstract
Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.
PMID:
24899449
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24899449
Equine influenza culture methods.
Chambers TM1, Reedy SE.
Author information
Abstract
Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.
PMID:
24899449
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24899449