tetano
Editor, Senior Moderator
Epidemiol Serv Saude
. 2022 Apr 15;31(1):e2021409.
doi: 10.1590/S1679-49742022000100002. eCollection 2022.
Self-collected nasopharyngeal swab and molecular test using pool testing as strategies to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, Brazil, 2021
[Article in Portuguese, English]
Nathalia Sernizon Guimarães[SUP] 1 [/SUP], Murilo Soares Costa[SUP] 1 [/SUP], Elaine Leandro Machado[SUP] 2 [/SUP], Hugo Itaru Sato[SUP] 3 [/SUP], Eduarda de Carvalho Maia E Amaral[SUP] 4 [/SUP], Rafaela Galvão Arivabene[SUP] 4 [/SUP], Karine Lima Lourenço[SUP] 3 [/SUP], Unaí Tupinambás[SUP] 5 [/SUP], Flávio Guimarães da Fonseca[SUP] 3 [/SUP], Ricardo Hiroshi Caldeira Takahashi[SUP] 6 [/SUP], Santuza Maria Ribeiro Teixeira[SUP] 3 [/SUP], Claudia Regina Lindgren Alves[SUP] 7 [/SUP]
Affiliations
Abstract
in English, Portuguese, Spanish
Objective: To show the feasibility of the combined use of self-collected nasopharyngeal swab and pool testing to detect SARS-CoV-2 in epidemiological surveys.
Methods: This experience included a sample of 154 students at the Universidade Federal de Minas Gerais, who performed self-collected nasopharyngeal swab in individual cabins and without supervision. The molecular test was performed using the pool testing technique.
Results: It took each person 5 minutes to collect the sample. An analysis was performed to detect endogenous RNA in 40 samples. The results showed that there were no failures resulting from self-collection. None of the pools detected the presence of viral RNA. The cost of molecular testing (RT-PCR), by pool testing, with samples obtained by self-collection was about ten times lower than the usual methods.
Conclusion: The strategies that were investigated proved to be economically feasible and valid for the research on SARS-CoV-2 in epidemiological surveys.
. 2022 Apr 15;31(1):e2021409.
doi: 10.1590/S1679-49742022000100002. eCollection 2022.
Self-collected nasopharyngeal swab and molecular test using pool testing as strategies to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, Brazil, 2021
[Article in Portuguese, English]
Nathalia Sernizon Guimarães[SUP] 1 [/SUP], Murilo Soares Costa[SUP] 1 [/SUP], Elaine Leandro Machado[SUP] 2 [/SUP], Hugo Itaru Sato[SUP] 3 [/SUP], Eduarda de Carvalho Maia E Amaral[SUP] 4 [/SUP], Rafaela Galvão Arivabene[SUP] 4 [/SUP], Karine Lima Lourenço[SUP] 3 [/SUP], Unaí Tupinambás[SUP] 5 [/SUP], Flávio Guimarães da Fonseca[SUP] 3 [/SUP], Ricardo Hiroshi Caldeira Takahashi[SUP] 6 [/SUP], Santuza Maria Ribeiro Teixeira[SUP] 3 [/SUP], Claudia Regina Lindgren Alves[SUP] 7 [/SUP]
Affiliations
- PMID: 35475998
- DOI: 10.1590/S1679-49742022000100002
Abstract
in English, Portuguese, Spanish
Objective: To show the feasibility of the combined use of self-collected nasopharyngeal swab and pool testing to detect SARS-CoV-2 in epidemiological surveys.
Methods: This experience included a sample of 154 students at the Universidade Federal de Minas Gerais, who performed self-collected nasopharyngeal swab in individual cabins and without supervision. The molecular test was performed using the pool testing technique.
Results: It took each person 5 minutes to collect the sample. An analysis was performed to detect endogenous RNA in 40 samples. The results showed that there were no failures resulting from self-collection. None of the pools detected the presence of viral RNA. The cost of molecular testing (RT-PCR), by pool testing, with samples obtained by self-collection was about ten times lower than the usual methods.
Conclusion: The strategies that were investigated proved to be economically feasible and valid for the research on SARS-CoV-2 in epidemiological surveys.