tetano
Editor, Senior Moderator
Anal Chem. 2014 Jan 29. [Epub ahead of print]
Enzyme-induced metallization as a signal amplification strategy for high sensitive colorimetric detection of avian influenza virus particles.
Zhou CH, Zhao JY, Pang DW, Zhang ZL.
Abstract
A novel colorimetric assay method based on enzyme-induced metallization has been proposed for detection of alkaline phosphatase (ALP), and it was further applied to high sensitive detection of avian influenza virus particles coupled with immunomagnetic separation. The enzyme-induced metallization-based color change strategy combined the amplification of the enzymatic reaction with the unique optical properties of metal nanoparticles (NPs), which could lead to a great enhancement in optical signal. The detection limit for ALP detection was 0.6 amol/50 μL which was 4-6 orders of magnitude more sensitive than other metal NP-based colorimetric method. Moreover, this technique was successfully employed to colorimetric viral immunosensor which could be applied to complex samples without complicated sample pretreatment and sophisticated instruments, and a detection limit as low as 17.5 pg mL-1 was achieved. This work not only provides a simple and sensitive sensing approach for ALP and virus detection, but also offers an effective signal enhancement strategy for development of high sensitive non-aggregation metal NP-based colorimetric assay method.
PMID:
24475750
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24475750
Enzyme-induced metallization as a signal amplification strategy for high sensitive colorimetric detection of avian influenza virus particles.
Zhou CH, Zhao JY, Pang DW, Zhang ZL.
Abstract
A novel colorimetric assay method based on enzyme-induced metallization has been proposed for detection of alkaline phosphatase (ALP), and it was further applied to high sensitive detection of avian influenza virus particles coupled with immunomagnetic separation. The enzyme-induced metallization-based color change strategy combined the amplification of the enzymatic reaction with the unique optical properties of metal nanoparticles (NPs), which could lead to a great enhancement in optical signal. The detection limit for ALP detection was 0.6 amol/50 μL which was 4-6 orders of magnitude more sensitive than other metal NP-based colorimetric method. Moreover, this technique was successfully employed to colorimetric viral immunosensor which could be applied to complex samples without complicated sample pretreatment and sophisticated instruments, and a detection limit as low as 17.5 pg mL-1 was achieved. This work not only provides a simple and sensitive sensing approach for ALP and virus detection, but also offers an effective signal enhancement strategy for development of high sensitive non-aggregation metal NP-based colorimetric assay method.
PMID:
24475750
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24475750