tetano
Editor, Senior Moderator
Biomed Environ Sci. 2016 Mar;29(3):224-8. doi: 10.3967/bes2016.028.
[h=1]Enhancement of Virus Replication in An Influenza A Virus NS1-Expresssing 293 Cell Line.[/h] Zhu WY[SUP]1[/SUP], Tao XY[SUP]1[/SUP], Lyu XJ[SUP]1[/SUP], Yu PC[SUP]1[/SUP], Lu ZZ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
Copyright ? 2016 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.
PMID: 27109134 [PubMed - in process] Free full text
[h=1]Enhancement of Virus Replication in An Influenza A Virus NS1-Expresssing 293 Cell Line.[/h] Zhu WY[SUP]1[/SUP], Tao XY[SUP]1[/SUP], Lyu XJ[SUP]1[/SUP], Yu PC[SUP]1[/SUP], Lu ZZ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
Copyright ? 2016 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.
PMID: 27109134 [PubMed - in process] Free full text