tetano
Editor, Senior Moderator
Bioengineered. 2012 Aug 24;4(1). [Epub ahead of print]
Engineering influenza viral vectors.
Li J, Ar?valo MT, Zeng M.
Source
Center of Excellence for Infectious Diseases; Department of Biomedical Sciences; Paul L. Foster School of Medicine; Texas Tech University Health Sciences Center; El Paso, TX USA.
Abstract
Influenza virus is a respiratory pathogen with a negative-sense, segmented RNA genome. Construction of recombinant influenza viruses in the laboratory was reported starting in the 1980s. Within a short period of time, pioneer researchers had devised methods that made it possible to construct influenza viral vectors from cDNA plasmid systems. Herein, we discuss the evolution of influenza virus reverse genetics, from helper virus-dependent systems, to helper virus-independent 17-plasmid systems, and all the way to 3- and 1- plasmid systems. Successes in the modification of different gene segments for various applications, including vaccine and gene therapies are highlighted.
PMID:
22922205
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22922205
Engineering influenza viral vectors.
Li J, Ar?valo MT, Zeng M.
Source
Center of Excellence for Infectious Diseases; Department of Biomedical Sciences; Paul L. Foster School of Medicine; Texas Tech University Health Sciences Center; El Paso, TX USA.
Abstract
Influenza virus is a respiratory pathogen with a negative-sense, segmented RNA genome. Construction of recombinant influenza viruses in the laboratory was reported starting in the 1980s. Within a short period of time, pioneer researchers had devised methods that made it possible to construct influenza viral vectors from cDNA plasmid systems. Herein, we discuss the evolution of influenza virus reverse genetics, from helper virus-dependent systems, to helper virus-independent 17-plasmid systems, and all the way to 3- and 1- plasmid systems. Successes in the modification of different gene segments for various applications, including vaccine and gene therapies are highlighted.
PMID:
22922205
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22922205