tetano
Editor, Senior Moderator
Endocrine
. 2020 Sep 1.
doi: 10.1007/s12020-020-02465-4. Online ahead of print.
Diabetes mellitus is a risk factor for prolonged SARS-CoV-2 viral shedding in lower respiratory tract samples of critically ill patients
Niccol? Buetti[SUP] 1 2 3 [/SUP], Pierpaolo Trimboli[SUP] 4 5 [/SUP], Timothy Mazzuchelli[SUP] 1 [/SUP], Elia Lo Priore[SUP] 6 7 [/SUP], Carlo Balmelli[SUP] 6 7 [/SUP], Alexandra Trkola[SUP] 8 [/SUP], Marco Conti[SUP] 1 [/SUP], Gladys Martinetti[SUP] 9 [/SUP], Luigia Elzi[SUP] 1 10 [/SUP], Alessandro Ceschi[SUP] 11 12 13 [/SUP], Vera Consonni[SUP] 1 [/SUP], Adam Ogna[SUP] 1 [/SUP], Valentina Forni-Ogna[SUP] 1 11 [/SUP], Enos Bernasconi[SUP] 7 [/SUP]
Affiliations
Abstract
Purpose: The length of time a critically ill coronavirus disease 2019 (COVID-19) patient remains infectious and should therefore be isolated remains unknown. This prospective study was undertaken in critically ill patients to evaluate the reliability of single negative real-time polymerase chain reaction (RT-PCR) in lower tracheal aspirates (LTA) in predicting a second negative test and to analyze clinical factors potentially influencing the viral shedding.
Methods: From April 9, 2020 onwards, intubated COVID-19 patients treated in the intensive care unit were systematically evaluated for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by RT-PCR of nasopharyngeal swabs and LTA. The time to negativity was defined as the time between the onset of symptoms and the viral clearance in LTA. In order to identify risk factors for prolonged viral shedding, we used univariate and multivariate Cox proportional hazards models.
Results: Forty-eight intubated SARS-CoV-2 patients were enrolled. Overall, we observed that the association of the first negative RT-PCR with a second negative result was 96.7%. Median viral shedding was 25 (IQR: 21.5-28) days since symptoms' onset. In the univariate Cox model analysis, type 2 diabetes mellitus was associated with a prolonged viral RNA shedding (hazard ratio
: 0.41, 95% CI: 0.06-3.11, p = 0.04). In the multivariate Cox model analysis, type 2 diabetes was associated with a prolonged viral RNA shedding (HR: 0.31, 95% CI: 0.11-0.89, p = 0.029).
Conclusion: Intubated patients with type 2 diabetes mellitus may have prolonged SARS-CoV-2 shedding. In critically ill COVID-19 patients, one negative LTA should be sufficient to assess and exclude infectivity.
Keywords: COVID-19; Infectivity; Intensive care unit; SARS-CoV-2; Type 2 diabetes mellitus; Viral shedding.
. 2020 Sep 1.
doi: 10.1007/s12020-020-02465-4. Online ahead of print.
Diabetes mellitus is a risk factor for prolonged SARS-CoV-2 viral shedding in lower respiratory tract samples of critically ill patients
Niccol? Buetti[SUP] 1 2 3 [/SUP], Pierpaolo Trimboli[SUP] 4 5 [/SUP], Timothy Mazzuchelli[SUP] 1 [/SUP], Elia Lo Priore[SUP] 6 7 [/SUP], Carlo Balmelli[SUP] 6 7 [/SUP], Alexandra Trkola[SUP] 8 [/SUP], Marco Conti[SUP] 1 [/SUP], Gladys Martinetti[SUP] 9 [/SUP], Luigia Elzi[SUP] 1 10 [/SUP], Alessandro Ceschi[SUP] 11 12 13 [/SUP], Vera Consonni[SUP] 1 [/SUP], Adam Ogna[SUP] 1 [/SUP], Valentina Forni-Ogna[SUP] 1 11 [/SUP], Enos Bernasconi[SUP] 7 [/SUP]
Affiliations
- PMID: 32870469
- DOI: 10.1007/s12020-020-02465-4
Abstract
Purpose: The length of time a critically ill coronavirus disease 2019 (COVID-19) patient remains infectious and should therefore be isolated remains unknown. This prospective study was undertaken in critically ill patients to evaluate the reliability of single negative real-time polymerase chain reaction (RT-PCR) in lower tracheal aspirates (LTA) in predicting a second negative test and to analyze clinical factors potentially influencing the viral shedding.
Methods: From April 9, 2020 onwards, intubated COVID-19 patients treated in the intensive care unit were systematically evaluated for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by RT-PCR of nasopharyngeal swabs and LTA. The time to negativity was defined as the time between the onset of symptoms and the viral clearance in LTA. In order to identify risk factors for prolonged viral shedding, we used univariate and multivariate Cox proportional hazards models.
Results: Forty-eight intubated SARS-CoV-2 patients were enrolled. Overall, we observed that the association of the first negative RT-PCR with a second negative result was 96.7%. Median viral shedding was 25 (IQR: 21.5-28) days since symptoms' onset. In the univariate Cox model analysis, type 2 diabetes mellitus was associated with a prolonged viral RNA shedding (hazard ratio
: 0.41, 95% CI: 0.06-3.11, p = 0.04). In the multivariate Cox model analysis, type 2 diabetes was associated with a prolonged viral RNA shedding (HR: 0.31, 95% CI: 0.11-0.89, p = 0.029).
Conclusion: Intubated patients with type 2 diabetes mellitus may have prolonged SARS-CoV-2 shedding. In critically ill COVID-19 patients, one negative LTA should be sufficient to assess and exclude infectivity.
Keywords: COVID-19; Infectivity; Intensive care unit; SARS-CoV-2; Type 2 diabetes mellitus; Viral shedding.