tetano
Editor, Senior Moderator
Emerg Microbes Infect
. 2022 Apr 14;1-49.
doi: 10.1080/22221751.2022.2065932. Online ahead of print.
N-chlorotaurine is highly active against respiratory viruses including SARS-CoV-2 (COVID-19) in vitro
Michaela Lackner[SUP] 1 [/SUP], Annika Rössler[SUP] 2 [/SUP], André Volland[SUP] 2 [/SUP], Marlena Nastassja Stadtmüller[SUP] 3 4 [/SUP], Brigitte Müllauer[SUP] 2 [/SUP], Zoltan Banki[SUP] 2 [/SUP], Johannes Ströhle[SUP] 1 [/SUP], Angela Luttick[SUP] 5 [/SUP], Jennifer Fenner[SUP] 5 [/SUP], Bettina Sarg[SUP] 6 [/SUP], Leopold Kremser[SUP] 6 [/SUP], Paul Tone[SUP] 7 [/SUP], Heribert Stoiber[SUP] 2 [/SUP], Dorothee von Laer[SUP] 2 [/SUP], Thorsten Wolff[SUP] 3 [/SUP], Carsten Schwarz[SUP] 8 [/SUP], Markus Nagl[SUP] 1 [/SUP]
Affiliations
Abstract
AbstractN-chlorotaurine (NCT) a long-lived oxidant generated by leukocytes, can be synthesized chemically and applied topically as an anti-infective to different body sites, including the lung via inhalation. Here, we demonstrate the activity of NCT against viruses causing acute respiratory tract infections, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza viruses, and respiratory syncytial virus (RSV).Virucidal activity of NCT was tested in plaque assays, confirmed by RT-qPCR assays. Attack on virus proteins was investigated by mass spectrometry.NCT revealed broad virucidal activity against all viruses tested at 37°C and pH 7. A significant reduction in infectious particles of SARS-CoV-2 isolates from early 2020 by 1 log[SUB]10[/SUB] was detected after 15 min of incubation in 1% NCT. Proteinaceous material simulating body fluids enhanced this activity by transchlorination mechanisms (1 -2 log[SUB]10[/SUB] reduction within 1-10 minutes). Tested SARS-CoV-2 variants B.1.1.7 (Alpha) und B.1.351 (Beta) showed a similar susceptibility. Influenza virus infectious particles were reduced by 3 log[SUB]10[/SUB] (H3N2) to 5 log[SUB]10[/SUB] (H1N1pdm), RSV by 4 log[SUB]10[/SUB]. Mass spectrometry of NCT-treated SARS-CoV-2 spike protein and 3C-like protease, influenza virus hemagglutinin and neuraminidase, and RSV fusion glycoprotein disclosed multiple sites of chlorination and oxidation as the molecular mechanism of action.Application of 1.0% NCT as a prophylactic and therapeutic strategy against acute viral respiratory tract infections deserves comprehensive clinical investigation.
Keywords: COVID-19; N-chlorotaurine; anti-infective; antiseptic; antiviral; influenza; respiratory syncytial virus; respiratory tract.
. 2022 Apr 14;1-49.
doi: 10.1080/22221751.2022.2065932. Online ahead of print.
N-chlorotaurine is highly active against respiratory viruses including SARS-CoV-2 (COVID-19) in vitro
Michaela Lackner[SUP] 1 [/SUP], Annika Rössler[SUP] 2 [/SUP], André Volland[SUP] 2 [/SUP], Marlena Nastassja Stadtmüller[SUP] 3 4 [/SUP], Brigitte Müllauer[SUP] 2 [/SUP], Zoltan Banki[SUP] 2 [/SUP], Johannes Ströhle[SUP] 1 [/SUP], Angela Luttick[SUP] 5 [/SUP], Jennifer Fenner[SUP] 5 [/SUP], Bettina Sarg[SUP] 6 [/SUP], Leopold Kremser[SUP] 6 [/SUP], Paul Tone[SUP] 7 [/SUP], Heribert Stoiber[SUP] 2 [/SUP], Dorothee von Laer[SUP] 2 [/SUP], Thorsten Wolff[SUP] 3 [/SUP], Carsten Schwarz[SUP] 8 [/SUP], Markus Nagl[SUP] 1 [/SUP]
Affiliations
- PMID: 35418279
- DOI: 10.1080/22221751.2022.2065932
Abstract
AbstractN-chlorotaurine (NCT) a long-lived oxidant generated by leukocytes, can be synthesized chemically and applied topically as an anti-infective to different body sites, including the lung via inhalation. Here, we demonstrate the activity of NCT against viruses causing acute respiratory tract infections, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza viruses, and respiratory syncytial virus (RSV).Virucidal activity of NCT was tested in plaque assays, confirmed by RT-qPCR assays. Attack on virus proteins was investigated by mass spectrometry.NCT revealed broad virucidal activity against all viruses tested at 37°C and pH 7. A significant reduction in infectious particles of SARS-CoV-2 isolates from early 2020 by 1 log[SUB]10[/SUB] was detected after 15 min of incubation in 1% NCT. Proteinaceous material simulating body fluids enhanced this activity by transchlorination mechanisms (1 -2 log[SUB]10[/SUB] reduction within 1-10 minutes). Tested SARS-CoV-2 variants B.1.1.7 (Alpha) und B.1.351 (Beta) showed a similar susceptibility. Influenza virus infectious particles were reduced by 3 log[SUB]10[/SUB] (H3N2) to 5 log[SUB]10[/SUB] (H1N1pdm), RSV by 4 log[SUB]10[/SUB]. Mass spectrometry of NCT-treated SARS-CoV-2 spike protein and 3C-like protease, influenza virus hemagglutinin and neuraminidase, and RSV fusion glycoprotein disclosed multiple sites of chlorination and oxidation as the molecular mechanism of action.Application of 1.0% NCT as a prophylactic and therapeutic strategy against acute viral respiratory tract infections deserves comprehensive clinical investigation.
Keywords: COVID-19; N-chlorotaurine; anti-infective; antiseptic; antiviral; influenza; respiratory syncytial virus; respiratory tract.