tetano
Editor, Senior Moderator
Emerg Microbes Infect
. 2023 Aug 5;2245931.
doi: 10.1080/22221751.2023.2245931. Online ahead of print. Characterizing the dynamics of BCR repertoire from repeated influenza vaccination
Guoqin Mai[SUP] 1 2 [/SUP], Chi Zhang[SUP] 1 2 [/SUP], Chunhong Lan[SUP] 3 4 5 [/SUP], Jie Zhang[SUP] 1 2 [/SUP], Yuanyuan Wang[SUP] 1 2 [/SUP], Kang Tang[SUP] 1 2 [/SUP], Jing Tang[SUP] 1 2 [/SUP], Jinfeng Zeng[SUP] 1 2 [/SUP], Yilin Chen[SUP] 1 2 [/SUP], Peiwen Cheng[SUP] 1 2 [/SUP], Shuning Liu[SUP] 1 2 [/SUP], Haoyu Long[SUP] 1 2 [/SUP], Qilan Wen[SUP] 1 2 [/SUP], Aqin Li[SUP] 1 2 [/SUP], Xuan Liu[SUP] 1 2 [/SUP], Ruitong Zhang[SUP] 1 2 [/SUP], Shuyang Xu[SUP] 1 2 [/SUP], Lin Liu[SUP] 1 2 [/SUP], Yanlan Niu[SUP] 1 2 [/SUP], Lan Yang[SUP] 1 2 [/SUP], Yihan Wang[SUP] 1 2 [/SUP], Di Yin[SUP] 1 2 [/SUP], Caijun Sun[SUP] 1 2 [/SUP], Yao-Qing Chen[SUP] 1 2 [/SUP], Wei Shen[SUP] 6 [/SUP], Zhenhai Zhang[SUP] 3 4 5 7 [/SUP], Xiangjun Du[SUP] 1 2 8 [/SUP]
Affiliations
Yearly epidemics of seasonal influenza cause an enormous disease burden around the globe. An understanding of the rules behind the immune response with repeated vaccination still presents a significant challenge, which would be helpful for optimizing the vaccination strategy. In this study, 34 healthy volunteers with 16 vaccinated were recruited, and the dynamics of the BCR repertoire for consecutive vaccinations in two seasons were tracked. In terms of diversity, length, network, V and J gene segments usage, somatic hypermutation (SHM) rate and isotype, it was found that the overall changes were stronger in the acute phase of the first vaccination than the second vaccination. However, the V gene segments of IGHV4-39, IGHV3-9, IGHV3-7 and IGHV1-69 were amplified in the acute phase of the first vaccination, with IGHV3-7 dominant. On the other hand, for the second vaccination, the changes were dominated by IGHV1-69, with potential for coding broad neutralizing antibody. Additional analysis indicates that the application of V gene segment for IGHV3-7 in the acute phase of the first vaccination was due to the elevated usage of isotypes IgM and IgG3. While for IGHV1-69 in the second vaccination, it was contributed by isotypes IgG1 and IgG2. Finally, 41 public BCR clusters were identified in the vaccine group, with both IGHV3-7 and IGHV1-69 were involved and representative complementarity determining region 3 (CDR3) motifs were characterized. This study provides insights into the immune response dynamics following repeated influenza vaccination in humans and can inform universal vaccine design and vaccine strategies in the future.
Keywords: influenza, vaccination, BCR repertoire, CDR3, V and J gene segments usage, public BCR cluster.
. 2023 Aug 5;2245931.
doi: 10.1080/22221751.2023.2245931. Online ahead of print. Characterizing the dynamics of BCR repertoire from repeated influenza vaccination
Guoqin Mai[SUP] 1 2 [/SUP], Chi Zhang[SUP] 1 2 [/SUP], Chunhong Lan[SUP] 3 4 5 [/SUP], Jie Zhang[SUP] 1 2 [/SUP], Yuanyuan Wang[SUP] 1 2 [/SUP], Kang Tang[SUP] 1 2 [/SUP], Jing Tang[SUP] 1 2 [/SUP], Jinfeng Zeng[SUP] 1 2 [/SUP], Yilin Chen[SUP] 1 2 [/SUP], Peiwen Cheng[SUP] 1 2 [/SUP], Shuning Liu[SUP] 1 2 [/SUP], Haoyu Long[SUP] 1 2 [/SUP], Qilan Wen[SUP] 1 2 [/SUP], Aqin Li[SUP] 1 2 [/SUP], Xuan Liu[SUP] 1 2 [/SUP], Ruitong Zhang[SUP] 1 2 [/SUP], Shuyang Xu[SUP] 1 2 [/SUP], Lin Liu[SUP] 1 2 [/SUP], Yanlan Niu[SUP] 1 2 [/SUP], Lan Yang[SUP] 1 2 [/SUP], Yihan Wang[SUP] 1 2 [/SUP], Di Yin[SUP] 1 2 [/SUP], Caijun Sun[SUP] 1 2 [/SUP], Yao-Qing Chen[SUP] 1 2 [/SUP], Wei Shen[SUP] 6 [/SUP], Zhenhai Zhang[SUP] 3 4 5 7 [/SUP], Xiangjun Du[SUP] 1 2 8 [/SUP]
Affiliations
- PMID: 37542407
- DOI: 10.1080/22221751.2023.2245931
Yearly epidemics of seasonal influenza cause an enormous disease burden around the globe. An understanding of the rules behind the immune response with repeated vaccination still presents a significant challenge, which would be helpful for optimizing the vaccination strategy. In this study, 34 healthy volunteers with 16 vaccinated were recruited, and the dynamics of the BCR repertoire for consecutive vaccinations in two seasons were tracked. In terms of diversity, length, network, V and J gene segments usage, somatic hypermutation (SHM) rate and isotype, it was found that the overall changes were stronger in the acute phase of the first vaccination than the second vaccination. However, the V gene segments of IGHV4-39, IGHV3-9, IGHV3-7 and IGHV1-69 were amplified in the acute phase of the first vaccination, with IGHV3-7 dominant. On the other hand, for the second vaccination, the changes were dominated by IGHV1-69, with potential for coding broad neutralizing antibody. Additional analysis indicates that the application of V gene segment for IGHV3-7 in the acute phase of the first vaccination was due to the elevated usage of isotypes IgM and IgG3. While for IGHV1-69 in the second vaccination, it was contributed by isotypes IgG1 and IgG2. Finally, 41 public BCR clusters were identified in the vaccine group, with both IGHV3-7 and IGHV1-69 were involved and representative complementarity determining region 3 (CDR3) motifs were characterized. This study provides insights into the immune response dynamics following repeated influenza vaccination in humans and can inform universal vaccine design and vaccine strategies in the future.
Keywords: influenza, vaccination, BCR repertoire, CDR3, V and J gene segments usage, public BCR cluster.