tetano
Editor, Senior Moderator
Emerg Microbes Infect
. 2023 Mar 29;2187245.
doi: 10.1080/22221751.2023.2187245. Online ahead of print.
Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing
Renyang Tong[SUP] 1 [/SUP], Lingjie Luo[SUP] 2 [/SUP], Yichao Zhao[SUP] 1 [/SUP], Mingze Sun[SUP] 1 [/SUP], Ronghong Li[SUP] 1 [/SUP], Jianmei Zhong[SUP] 1 [/SUP], Yifan Chen[SUP] 1 [/SUP], Liuhua Hu[SUP] 1 [/SUP], Zheng Li[SUP] 1 [/SUP], Jianfeng Shi[SUP] 1 [/SUP], Yuyan Lyu[SUP] 1 [/SUP], Li Hu[SUP] 1 [/SUP], Xiao Guo[SUP] 1 [/SUP], Qi Liu[SUP] 1 [/SUP], Tian Shuang[SUP] 1 [/SUP], Chenjie Zhang[SUP] 1 [/SUP], Ancai Yuan[SUP] 1 [/SUP], Lingyue Sun[SUP] 1 [/SUP], Zheng Zhang[SUP] 3 [/SUP], Kun Qian[SUP] 1 4 [/SUP], Lei Chen[SUP] 1 [/SUP], Wei Lin[SUP] 1 [/SUP], Alex F Chen[SUP] 5 [/SUP], Feng Wang[SUP] 2 [/SUP], Jun Pu[SUP] 1 [/SUP]
Affiliations
Abstract
Over 3 billion doses of inactivated vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been administered globally. However, our understanding of the immune cell functional transcription and T cell receptor (TCR)/B cell receptor (BCR) repertoire dynamics following inactivated SARS-CoV-2 vaccination remains poorly understood. Here, we performed single-cell RNA and TCR/BCR sequencing on peripheral blood mononuclear cells at four time points after immunization with the inactivated SARS-CoV-2 vaccine BBIBP-CorV. Our analysis revealed an enrichment of monocytes, central memory CD4[SUP]+[/SUP] T cells, type 2 helper T cells and memory B cells following vaccination. Single-cell TCR-seq and RNA-seq comminating analysis identified a clonal expansion of CD4[SUP]+[/SUP] T cells (but not CD8[SUP]+[/SUP] T cells) following a booster vaccination that corresponded to a decrease in the TCR diversity of central memory CD4[SUP]+[/SUP] T cells and type 2 helper T cells. Importantly, these TCR repertoire changes and CD4[SUP]+[/SUP] T cell differentiation were correlated with the biased VJ gene usage of BCR and the antibody-producing function of B cells post-vaccination. Finally, we compared the functional transcription and repertoire dynamics in immune cells elicited by vaccination and SARS-CoV-2 infection to explore the immune responses under different stimuli. Our data provide novel molecular and cellular evidence for the CD4[SUP]+[/SUP] T cell-dependent antibody response induced by inactivated vaccine BBIBP-CorV. This information is urgently needed to develop new prevention and control strategies for SARS-CoV-2 infection. (ClinicalTrials.gov Identifier: NCT04871932).Trial registration: ClinicalTrials.gov identifier: NCT04871932..
Keywords: BBIBP-CorV; Inactivated SARS-CoV-2 vaccine; Peripheral blood mononuclear cells; Single-cell RNA sequencing; Single-cell TCR/BCR sequencing.
. 2023 Mar 29;2187245.
doi: 10.1080/22221751.2023.2187245. Online ahead of print.
Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing
Renyang Tong[SUP] 1 [/SUP], Lingjie Luo[SUP] 2 [/SUP], Yichao Zhao[SUP] 1 [/SUP], Mingze Sun[SUP] 1 [/SUP], Ronghong Li[SUP] 1 [/SUP], Jianmei Zhong[SUP] 1 [/SUP], Yifan Chen[SUP] 1 [/SUP], Liuhua Hu[SUP] 1 [/SUP], Zheng Li[SUP] 1 [/SUP], Jianfeng Shi[SUP] 1 [/SUP], Yuyan Lyu[SUP] 1 [/SUP], Li Hu[SUP] 1 [/SUP], Xiao Guo[SUP] 1 [/SUP], Qi Liu[SUP] 1 [/SUP], Tian Shuang[SUP] 1 [/SUP], Chenjie Zhang[SUP] 1 [/SUP], Ancai Yuan[SUP] 1 [/SUP], Lingyue Sun[SUP] 1 [/SUP], Zheng Zhang[SUP] 3 [/SUP], Kun Qian[SUP] 1 4 [/SUP], Lei Chen[SUP] 1 [/SUP], Wei Lin[SUP] 1 [/SUP], Alex F Chen[SUP] 5 [/SUP], Feng Wang[SUP] 2 [/SUP], Jun Pu[SUP] 1 [/SUP]
Affiliations
- PMID: 36987861
- DOI: 10.1080/22221751.2023.2187245
Abstract
Over 3 billion doses of inactivated vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been administered globally. However, our understanding of the immune cell functional transcription and T cell receptor (TCR)/B cell receptor (BCR) repertoire dynamics following inactivated SARS-CoV-2 vaccination remains poorly understood. Here, we performed single-cell RNA and TCR/BCR sequencing on peripheral blood mononuclear cells at four time points after immunization with the inactivated SARS-CoV-2 vaccine BBIBP-CorV. Our analysis revealed an enrichment of monocytes, central memory CD4[SUP]+[/SUP] T cells, type 2 helper T cells and memory B cells following vaccination. Single-cell TCR-seq and RNA-seq comminating analysis identified a clonal expansion of CD4[SUP]+[/SUP] T cells (but not CD8[SUP]+[/SUP] T cells) following a booster vaccination that corresponded to a decrease in the TCR diversity of central memory CD4[SUP]+[/SUP] T cells and type 2 helper T cells. Importantly, these TCR repertoire changes and CD4[SUP]+[/SUP] T cell differentiation were correlated with the biased VJ gene usage of BCR and the antibody-producing function of B cells post-vaccination. Finally, we compared the functional transcription and repertoire dynamics in immune cells elicited by vaccination and SARS-CoV-2 infection to explore the immune responses under different stimuli. Our data provide novel molecular and cellular evidence for the CD4[SUP]+[/SUP] T cell-dependent antibody response induced by inactivated vaccine BBIBP-CorV. This information is urgently needed to develop new prevention and control strategies for SARS-CoV-2 infection. (ClinicalTrials.gov Identifier: NCT04871932).Trial registration: ClinicalTrials.gov identifier: NCT04871932..
Keywords: BBIBP-CorV; Inactivated SARS-CoV-2 vaccine; Peripheral blood mononuclear cells; Single-cell RNA sequencing; Single-cell TCR/BCR sequencing.