tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2021 Dec;27(12):3052-3062.
doi: 10.3201/eid2712.210344.
Surface‒Aerosol Stability and Pathogenicity of Diverse Middle East Respiratory Syndrome Coronavirus Strains, 2012‒2018
Neeltje van Doremalen, Michael Letko, Robert J Fischer, Trenton Bushmaker, Jonathan Schulz, Claude K Yinda, Stephanie N Seifert, Nam Joong Kim, Maged G Hemida, Ghazi Kayali, Wan Beom Park, Ranawaka A P M Perera, Azaibi Tamin, Natalie J Thornburg, Suxiang Tong, Krista Queen, Maria D van Kerkhove, Young Ki Choi, Myoung-Don Oh, Abdullah M Assiri, Malik Peiris, Susan I Gerber, Vincent J Munster
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infects humans and dromedary camels and is responsible for an ongoing outbreak of severe respiratory illness in humans in the Middle East. Although some mutations found in camel-derived MERS-CoV strains have been characterized, most natural variation found across MERS-CoV isolates remains unstudied. We report on the environmental stability, replication kinetics, and pathogenicity of several diverse isolates of MERS-CoV, as well as isolates of severe acute respiratory syndrome coronavirus 2, to serve as a basis of comparison with other stability studies. Although most MERS-CoV isolates had similar stability and pathogenicity in our experiments, the camel-derived isolate C/KSA/13 had reduced surface stability, and another camel isolate, C/BF/15, had reduced pathogenicity in a small animal model. These results suggest that although betacoronaviruses might have similar environmental stability profiles, individual variation can influence this phenotype, underscoring the need for continual global viral surveillance.
Keywords: MERS-CoV; Middle East respiratory syndrome coronavirus; SARS-CoV-2; coronavirus; pathogenicity; respiratory infections; severe acute respiratory syndrome coronavirus 2; strains; surface‒aerosol stability; viruses; zoonoses.
. 2021 Dec;27(12):3052-3062.
doi: 10.3201/eid2712.210344.
Surface‒Aerosol Stability and Pathogenicity of Diverse Middle East Respiratory Syndrome Coronavirus Strains, 2012‒2018
Neeltje van Doremalen, Michael Letko, Robert J Fischer, Trenton Bushmaker, Jonathan Schulz, Claude K Yinda, Stephanie N Seifert, Nam Joong Kim, Maged G Hemida, Ghazi Kayali, Wan Beom Park, Ranawaka A P M Perera, Azaibi Tamin, Natalie J Thornburg, Suxiang Tong, Krista Queen, Maria D van Kerkhove, Young Ki Choi, Myoung-Don Oh, Abdullah M Assiri, Malik Peiris, Susan I Gerber, Vincent J Munster
- PMID: 34808078
- DOI: 10.3201/eid2712.210344
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infects humans and dromedary camels and is responsible for an ongoing outbreak of severe respiratory illness in humans in the Middle East. Although some mutations found in camel-derived MERS-CoV strains have been characterized, most natural variation found across MERS-CoV isolates remains unstudied. We report on the environmental stability, replication kinetics, and pathogenicity of several diverse isolates of MERS-CoV, as well as isolates of severe acute respiratory syndrome coronavirus 2, to serve as a basis of comparison with other stability studies. Although most MERS-CoV isolates had similar stability and pathogenicity in our experiments, the camel-derived isolate C/KSA/13 had reduced surface stability, and another camel isolate, C/BF/15, had reduced pathogenicity in a small animal model. These results suggest that although betacoronaviruses might have similar environmental stability profiles, individual variation can influence this phenotype, underscoring the need for continual global viral surveillance.
Keywords: MERS-CoV; Middle East respiratory syndrome coronavirus; SARS-CoV-2; coronavirus; pathogenicity; respiratory infections; severe acute respiratory syndrome coronavirus 2; strains; surface‒aerosol stability; viruses; zoonoses.