tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2022 Feb 9;28(3).
doi: 10.3201/eid2803.211735. Online ahead of print.
Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta
Sarah Reagan-Steiner, Julu Bhatnagar, Roosecelis B Martines, Nicholas S Milligan, Carly Gisondo, Frank B Williams, Elizabeth Lee, Lindsey Estetter, Hannah Bullock, Cynthia S Goldsmith, Pamela Fair, Julie Hand, Gillian Richardson, Kate R Woodworth, Titilope Oduyebo, Romeo R Galang, Rebecca Phillips, Elizaveta Belyaeva, Xiao-Ming Yin, Dana Meaney-Delman, Timothy M Uyeki, Drucilla J Roberts, Sherif R Zaki
Abstract
Severe coronavirus disease in neonates is rare. We analyzed clinical, laboratory, and autopsy findings from a neonate in the United States who was delivered at 25 weeks of gestation and died 4 days after birth; the mother had asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and preeclampsia. We observed severe diffuse alveolar damage and localized SARS-CoV-2 by immunohistochemistry, in situ hybridization, and electron microscopy of the lungs of the neonate. We localized SARS-CoV-2 RNA in neonatal heart and liver vascular endothelium by using in situ hybridization and detected SARS-CoV-2 RNA in neonatal and placental tissues by using reverse transcription PCR. Subgenomic reverse transcription PCR suggested viral replication in lung/airway, heart, and liver. These findings indicate that in utero SARS-CoV-2 transmission contributed to this neonatal death.
Keywords: 2019 novel coronavirus disease; COVID-19; SARS-CoV-2; autopsy; coronavirus disease; infant; newborn; pregnancy; respiratory infections; severe acute respiratory syndrome coronavirus 2; vertical transmission; viruses; zoonoses.
. 2022 Feb 9;28(3).
doi: 10.3201/eid2803.211735. Online ahead of print.
Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta
Sarah Reagan-Steiner, Julu Bhatnagar, Roosecelis B Martines, Nicholas S Milligan, Carly Gisondo, Frank B Williams, Elizabeth Lee, Lindsey Estetter, Hannah Bullock, Cynthia S Goldsmith, Pamela Fair, Julie Hand, Gillian Richardson, Kate R Woodworth, Titilope Oduyebo, Romeo R Galang, Rebecca Phillips, Elizaveta Belyaeva, Xiao-Ming Yin, Dana Meaney-Delman, Timothy M Uyeki, Drucilla J Roberts, Sherif R Zaki
- PMID: 35138244
- DOI: 10.3201/eid2803.211735
Abstract
Severe coronavirus disease in neonates is rare. We analyzed clinical, laboratory, and autopsy findings from a neonate in the United States who was delivered at 25 weeks of gestation and died 4 days after birth; the mother had asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and preeclampsia. We observed severe diffuse alveolar damage and localized SARS-CoV-2 by immunohistochemistry, in situ hybridization, and electron microscopy of the lungs of the neonate. We localized SARS-CoV-2 RNA in neonatal heart and liver vascular endothelium by using in situ hybridization and detected SARS-CoV-2 RNA in neonatal and placental tissues by using reverse transcription PCR. Subgenomic reverse transcription PCR suggested viral replication in lung/airway, heart, and liver. These findings indicate that in utero SARS-CoV-2 transmission contributed to this neonatal death.
Keywords: 2019 novel coronavirus disease; COVID-19; SARS-CoV-2; autopsy; coronavirus disease; infant; newborn; pregnancy; respiratory infections; severe acute respiratory syndrome coronavirus 2; vertical transmission; viruses; zoonoses.