tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2026 Oct;32(10):1636-1644.
doi: 10.3201/eid3210.260499.
Carlos Abelardo Dos Santos, Jialu Li, Pauline M van Diemen, Andrew McMahon, Benjamin C Mollett, Andrew M Ramsay, Meshach M Maina, Joe James, Helen E Everett, Janet M Daly, Nicole C Robb
The Orthomyxoviridae family includes influenza D virus (IDV), an emerging pathogen primarily affecting cattle and swine; there is evidence of cross-species transmission and potential zoonotic risk. Although active human infections have yet to be confirmed, high seroprevalence in cattle-exposed populations highlights the need for continued surveillance. We developed and validated a rapid, field-deployable reverse transcription loop-mediated isothermal amplification assay for IDV detection; specificity was 99.2% and sensitivity ranged from 95.6% (cycle quantification <30) to 81.8% (cycle quantification <40). This method offers a cost-effective, accessible alternative to quantitative reverse transcription PCR, enabling improved monitoring of IDV and reinforcing preparedness for emerging influenza threats.
Keywords: RT-LAMP; United Kingdom; influenza; influenza D; molecular diagnostics; viruses; zoonoses.
. 2026 Oct;32(10):1636-1644.
doi: 10.3201/eid3210.260499.
Detection of Influenza D Virus using Reverse Transcription Loop-Mediated Isothermal Amplification
Carlos Abelardo Dos Santos, Jialu Li, Pauline M van Diemen, Andrew McMahon, Benjamin C Mollett, Andrew M Ramsay, Meshach M Maina, Joe James, Helen E Everett, Janet M Daly, Nicole C Robb
- PMID: 42811284
- DOI: 10.3201/eid3210.260499
Abstract
The Orthomyxoviridae family includes influenza D virus (IDV), an emerging pathogen primarily affecting cattle and swine; there is evidence of cross-species transmission and potential zoonotic risk. Although active human infections have yet to be confirmed, high seroprevalence in cattle-exposed populations highlights the need for continued surveillance. We developed and validated a rapid, field-deployable reverse transcription loop-mediated isothermal amplification assay for IDV detection; specificity was 99.2% and sensitivity ranged from 95.6% (cycle quantification <30) to 81.8% (cycle quantification <40). This method offers a cost-effective, accessible alternative to quantitative reverse transcription PCR, enabling improved monitoring of IDV and reinforcing preparedness for emerging influenza threats.
Keywords: RT-LAMP; United Kingdom; influenza; influenza D; molecular diagnostics; viruses; zoonoses.