tetano
Editor, Senior Moderator
Anal Chem. 2017 Oct 20. doi: 10.1021/acs.analchem.7b02784. [Epub ahead of print]
[h=1]Electrochemical Conversion of Fe3O4 Magnetic Nanoparticles to Electroactive Prussian Blue Analogues for Novel Self-Sacrificial-Label Biosensing of Avian Influenza Virus H5N1.[/h] Zhang Q, Li L, Qiao Z, Lei C, Fu Y, Xie Q, Yao S, Li Y, Ying Y.
[h=3]Abstract[/h] A serious impetus always exists to exploit new methods to enrich the prospect of nanomaterials. Here, we report electrochemical conversion (ECC) of magnetic nanoparticles (MNPs) to electroactive Prussian blue (PB) analogues accompanying with multiple interfacial effects and its exploitation for novel label self-sacrificial biosensing strategy. The ECC method involves a high potential step to create strong acidic condition by splitting H2O to release Fe3+ from the MNPs, and then a low potential step leading to the reduction of co-existing K3Fe(CN)6 and Fe3+ to K4Fe(CN)6 and Fe2+, respectively, which react to form PB analogues. Other than conventional solid/liquid electrochemical interface that needs supply of reactants by the transportation from bulk solution and requires additional template to generate porosity, the proposed method introduces MNPs on the electrode surface and makes them as natural nano-templates and nano-confined-sources of reactants. Therefore, the method presents a series of interesting surficial "templating", "generation-confinement", and "refreshing" effects/modes, which benefit the produced PB with higher porosity and electrochemical activity, and three-magnitude-lower requirement on reactant concentration compared with conventional method. Based on the ECC method, a sandwich immunosensor is designed using MNPs as self-sacrificial labels and using the formed PB for signal amplification. Taking full advantages of the high abundance of Fe in MNPs and three surficial effects, the ECC method endows the biosensing technology with high sensitivity and a limit of detection down to 0.0022 HAU, which is better than those of most reported analogues. The ECC method may lead to new direction for the application of nanomaterials and new electrochemistry modes.
PMID: 29053256 DOI: 10.1021/acs.analchem.7b02784
[h=1]Electrochemical Conversion of Fe3O4 Magnetic Nanoparticles to Electroactive Prussian Blue Analogues for Novel Self-Sacrificial-Label Biosensing of Avian Influenza Virus H5N1.[/h] Zhang Q, Li L, Qiao Z, Lei C, Fu Y, Xie Q, Yao S, Li Y, Ying Y.
[h=3]Abstract[/h] A serious impetus always exists to exploit new methods to enrich the prospect of nanomaterials. Here, we report electrochemical conversion (ECC) of magnetic nanoparticles (MNPs) to electroactive Prussian blue (PB) analogues accompanying with multiple interfacial effects and its exploitation for novel label self-sacrificial biosensing strategy. The ECC method involves a high potential step to create strong acidic condition by splitting H2O to release Fe3+ from the MNPs, and then a low potential step leading to the reduction of co-existing K3Fe(CN)6 and Fe3+ to K4Fe(CN)6 and Fe2+, respectively, which react to form PB analogues. Other than conventional solid/liquid electrochemical interface that needs supply of reactants by the transportation from bulk solution and requires additional template to generate porosity, the proposed method introduces MNPs on the electrode surface and makes them as natural nano-templates and nano-confined-sources of reactants. Therefore, the method presents a series of interesting surficial "templating", "generation-confinement", and "refreshing" effects/modes, which benefit the produced PB with higher porosity and electrochemical activity, and three-magnitude-lower requirement on reactant concentration compared with conventional method. Based on the ECC method, a sandwich immunosensor is designed using MNPs as self-sacrificial labels and using the formed PB for signal amplification. Taking full advantages of the high abundance of Fe in MNPs and three surficial effects, the ECC method endows the biosensing technology with high sensitivity and a limit of detection down to 0.0022 HAU, which is better than those of most reported analogues. The ECC method may lead to new direction for the application of nanomaterials and new electrochemistry modes.
PMID: 29053256 DOI: 10.1021/acs.analchem.7b02784