tetano
Editor, Senior Moderator
Anal Chim Acta. 2017 Aug 1;979:51-57. doi: 10.1016/j.aca.2017.05.010. Epub 2017 May 26.
[h=1]Electrochemical biosensing of influenza A subtype genome based on meso/macroporous cobalt (II) oxide nanoflakes-applied to human samples.[/h] Mohammadi J[SUP]1[/SUP], Moattari A[SUP]2[/SUP], Sattarahmady N[SUP]3[/SUP], Pirbonyeh N[SUP]2[/SUP], Yadegari H[SUP]4[/SUP], Heli H[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Meso/macroporous cobalt (II) oxide nanoflakes were electrodeposited in a one-step process in the presence of N-methylpyrrolidone. On the surface of nanoflakes, a specific single stranded DNA sequence from the genome of influenza A subtype was then immobilized to fabricate an electrochemical biosensor. Hybridization of the biosensor with complementary, non-complementary and base-mismatch sequences was electrochemically detected. The biosensor was also employed to detect complementary DNA of viral RNA in culture and human samples. The biosensor could detect the complementary sequence with a detection limit of 86.4 amol L[SUP]-1[/SUP] and a linear concentration range of 1.0 fmol L[SUP]-1[/SUP] to 1.0 nmol L[SUP]-1[/SUP]. It also detected a complementary DNA sequence converted from viral RNA with a detection limit of 0.28 ng μL[SUP]-1[/SUP] in a linear concentration range of 0.5-10 ng μL[SUP]-1[/SUP]. Low detection limit, simple method of preparation of the transducer and no needing any DNA strand modification and tag are the principal advantages of the biosensor.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] CoO; Cobaltous oxide; DNA biosensor; Genosensor; Influenza; Nanoflake
PMID: 28599709 DOI: 10.1016/j.aca.2017.05.010
[h=1]Electrochemical biosensing of influenza A subtype genome based on meso/macroporous cobalt (II) oxide nanoflakes-applied to human samples.[/h] Mohammadi J[SUP]1[/SUP], Moattari A[SUP]2[/SUP], Sattarahmady N[SUP]3[/SUP], Pirbonyeh N[SUP]2[/SUP], Yadegari H[SUP]4[/SUP], Heli H[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Meso/macroporous cobalt (II) oxide nanoflakes were electrodeposited in a one-step process in the presence of N-methylpyrrolidone. On the surface of nanoflakes, a specific single stranded DNA sequence from the genome of influenza A subtype was then immobilized to fabricate an electrochemical biosensor. Hybridization of the biosensor with complementary, non-complementary and base-mismatch sequences was electrochemically detected. The biosensor was also employed to detect complementary DNA of viral RNA in culture and human samples. The biosensor could detect the complementary sequence with a detection limit of 86.4 amol L[SUP]-1[/SUP] and a linear concentration range of 1.0 fmol L[SUP]-1[/SUP] to 1.0 nmol L[SUP]-1[/SUP]. It also detected a complementary DNA sequence converted from viral RNA with a detection limit of 0.28 ng μL[SUP]-1[/SUP] in a linear concentration range of 0.5-10 ng μL[SUP]-1[/SUP]. Low detection limit, simple method of preparation of the transducer and no needing any DNA strand modification and tag are the principal advantages of the biosensor.
Copyright ? 2017 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] CoO; Cobaltous oxide; DNA biosensor; Genosensor; Influenza; Nanoflake
PMID: 28599709 DOI: 10.1016/j.aca.2017.05.010