tetano
Editor, Senior Moderator
Expert Rev Vaccines. 2015 Mar 27:1-10. [Epub ahead of print]
[h=1]Efficient vaccine against pandemic influenza: combining DNA vaccination and targeted delivery to MHC class II molecules.[/h] Gr?deland G[SUP]1[/SUP], Bogen B.
[h=3]Author information[/h]
[h=3]Abstract[/h] There are two major limitations to vaccine preparedness in the event of devastating influenza pandemics: the time needed to generate a vaccine and rapid generation of sufficient amounts. DNA vaccination could represent a solution to these problems, but efficacy needs to be enhanced. In a separate line of research, it has been established that targeting of vaccine molecules to antigen-presenting cells enhances immune responses. We have combined the two principles by constructing DNA vaccines that encode bivalent fusion proteins; these target hemagglutinin to MHC class II molecules on antigen-presenting cells. Such DNA vaccines rapidly induce hemagglutinin-specific antibodies and T cell responses in immunized mice. Responses are long-lasting and protect mice against challenge with influenza virus. In a pandemic situation, targeted DNA vaccines could be produced and tested within a month. The novel DNA vaccines could represent a solution to pandemic preparedness in the advent of novel influenza pandemics.
[h=4]KEYWORDS:[/h] APC targeting; DNA vaccine; MHC II molecules; antibodies; influenza; vaccine
PMID: 25818107 [PubMed - as supplied by publisher]
[h=1]Efficient vaccine against pandemic influenza: combining DNA vaccination and targeted delivery to MHC class II molecules.[/h] Gr?deland G[SUP]1[/SUP], Bogen B.
[h=3]Author information[/h]
[h=3]Abstract[/h] There are two major limitations to vaccine preparedness in the event of devastating influenza pandemics: the time needed to generate a vaccine and rapid generation of sufficient amounts. DNA vaccination could represent a solution to these problems, but efficacy needs to be enhanced. In a separate line of research, it has been established that targeting of vaccine molecules to antigen-presenting cells enhances immune responses. We have combined the two principles by constructing DNA vaccines that encode bivalent fusion proteins; these target hemagglutinin to MHC class II molecules on antigen-presenting cells. Such DNA vaccines rapidly induce hemagglutinin-specific antibodies and T cell responses in immunized mice. Responses are long-lasting and protect mice against challenge with influenza virus. In a pandemic situation, targeted DNA vaccines could be produced and tested within a month. The novel DNA vaccines could represent a solution to pandemic preparedness in the advent of novel influenza pandemics.
[h=4]KEYWORDS:[/h] APC targeting; DNA vaccine; MHC II molecules; antibodies; influenza; vaccine
PMID: 25818107 [PubMed - as supplied by publisher]