tetano
Editor, Senior Moderator
J Virol. 2013 Jun 26. [Epub ahead of print]
Efficacy of Parainfluenza virus 5 mutants expressing HA from H5N1 influenza A virus in mice.
Li Z, Gabbard JD, Mooney A, Chen Z, Tompkins SM, He B.
Source
Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA 30602.
Abstract
Parainfluenza virus 5 (PIV5) is a promising viral vector for vaccine development. PIV5 is safe, stable, efficacious, cost-effective to produce and, most interestingly, it overcomes pre-existing anti-vector immunity. We have recently reported that PIV5 expressing the hemagglutinin (HA) from highly pathogenic avian influenza (HPAI) virus H5N1 (PIV5-H5) provides sterilizing immunity against lethal dose of HPAI H5N1 infection in mice. It is thought that induction of apoptosis can lead to enhanced antigen presentation. Previously, we have shown that deleting the SH gene and the conserved C-terminus of the V gene in PIV5 results in mutant viruses (PIV5ΔSH and PIV5VΔC) that enhance induction of apoptosis. In this work we have inserted the HA gene of H5N1 into PIV5ΔSH (PIV5ΔSH-H5) or PIV5VΔC (PIV5VΔC-H5) and compared their efficacies as vaccine candidates to PIV5-H5. We have found that PIV5ΔSH-H5 induced the highest levels of anti-HA antibodies, the strongest T cell responses and the best protection against an H5N1 lethal challenge in mice. These results suggest that PIV5ΔSH is a better vaccine vector than wild type PIV5.
PMID:
23804633
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23804633
Efficacy of Parainfluenza virus 5 mutants expressing HA from H5N1 influenza A virus in mice.
Li Z, Gabbard JD, Mooney A, Chen Z, Tompkins SM, He B.
Source
Department of Infectious Diseases, University of Georgia College of Veterinary Medicine, Athens, GA 30602.
Abstract
Parainfluenza virus 5 (PIV5) is a promising viral vector for vaccine development. PIV5 is safe, stable, efficacious, cost-effective to produce and, most interestingly, it overcomes pre-existing anti-vector immunity. We have recently reported that PIV5 expressing the hemagglutinin (HA) from highly pathogenic avian influenza (HPAI) virus H5N1 (PIV5-H5) provides sterilizing immunity against lethal dose of HPAI H5N1 infection in mice. It is thought that induction of apoptosis can lead to enhanced antigen presentation. Previously, we have shown that deleting the SH gene and the conserved C-terminus of the V gene in PIV5 results in mutant viruses (PIV5ΔSH and PIV5VΔC) that enhance induction of apoptosis. In this work we have inserted the HA gene of H5N1 into PIV5ΔSH (PIV5ΔSH-H5) or PIV5VΔC (PIV5VΔC-H5) and compared their efficacies as vaccine candidates to PIV5-H5. We have found that PIV5ΔSH-H5 induced the highest levels of anti-HA antibodies, the strongest T cell responses and the best protection against an H5N1 lethal challenge in mice. These results suggest that PIV5ΔSH is a better vaccine vector than wild type PIV5.
PMID:
23804633
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23804633