tetano
Editor, Senior Moderator
Veterinary Microbiology
Available online 9 July 2012
In Press, Accepted Manuscript ? Note to users
Cover image
Efficacy of a High-yield Attenuated Vaccine Strain Wholly Derived from Avian Influenza Viruses by Use of Reverse Genetics
Ming Liu E-mail the corresponding author,
Chun-Guo Liu,
Yun ZhangCorresponding author contact information, E-mail the corresponding author,
Wei-Lin Shi,
Wei Wang,
Yan-Yun Liu
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS, Harbin 150001, China
Received 30 March 2012. Revised 30 June 2012. Accepted 2 July 2012. Available online 9 July 2012.
http://dx.doi.org/10.1016/j.vetmic.2012.07.005, How to Cite or Link Using DOI
Cited by in Scopus (0)
Abstract
The preparation of high-yield influenza H5N1 vaccine strains is challenging for researchers and manufacturers. Here, we used reverse genetics to generate a high-yield avian influenza vaccine strain based on a novel avian influenza virus. A high-yield attenuated recombinant H5N3 virus (rH5N3-DL) was prepared from the HA gene of A/Goose/Anhui/08 (H5N1), modified by deletion of the multiple basic amino acids at the cleavage site, the NA gene from A/Duck/Germany/1215/73 (H2N3), and the six internal genes from the high-yield A/Goose/Dalian/3/01 (H9N2) virus. rH5N3-DL grew to high HA titers (1:2048) in eggs, eight times those of the parental H5N1 virus, and four times higher than that of rH5N3-PR8(six internal genes from the high-yield PR8). Infection tests demonstrated that rH5N3-DL was avirulent in chickens, chicken embryos, and mice. rH5N3-DL-vaccinated chickens were fully protected against the morbidity and mortality of a lethal challenge with homologous A/Goose/Anhui/08, but only 80% of chickens were protected after challenge with heterologous A/Goose/Guangdong/1/96. The N3 neuraminidase marker distinguishes rH5N3-DL-vaccinated from H5N1-infected animals. rH5N3-DL is thus a promising vaccine candidate to combat highly pathogenic avian influenza virus infections. The A/Goose/Dalian/3/01 virus could be a promising candidate as providing internal genes donors with high-yield properties in reverse-genetics system and might be applicable for future avian influenza vaccine development.
http://www.sciencedirect.com/science/article/pii/S0378113512003902
Available online 9 July 2012
In Press, Accepted Manuscript ? Note to users
Cover image
Efficacy of a High-yield Attenuated Vaccine Strain Wholly Derived from Avian Influenza Viruses by Use of Reverse Genetics
Ming Liu E-mail the corresponding author,
Chun-Guo Liu,
Yun ZhangCorresponding author contact information, E-mail the corresponding author,
Wei-Lin Shi,
Wei Wang,
Yan-Yun Liu
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS, Harbin 150001, China
Received 30 March 2012. Revised 30 June 2012. Accepted 2 July 2012. Available online 9 July 2012.
http://dx.doi.org/10.1016/j.vetmic.2012.07.005, How to Cite or Link Using DOI
Cited by in Scopus (0)
Abstract
The preparation of high-yield influenza H5N1 vaccine strains is challenging for researchers and manufacturers. Here, we used reverse genetics to generate a high-yield avian influenza vaccine strain based on a novel avian influenza virus. A high-yield attenuated recombinant H5N3 virus (rH5N3-DL) was prepared from the HA gene of A/Goose/Anhui/08 (H5N1), modified by deletion of the multiple basic amino acids at the cleavage site, the NA gene from A/Duck/Germany/1215/73 (H2N3), and the six internal genes from the high-yield A/Goose/Dalian/3/01 (H9N2) virus. rH5N3-DL grew to high HA titers (1:2048) in eggs, eight times those of the parental H5N1 virus, and four times higher than that of rH5N3-PR8(six internal genes from the high-yield PR8). Infection tests demonstrated that rH5N3-DL was avirulent in chickens, chicken embryos, and mice. rH5N3-DL-vaccinated chickens were fully protected against the morbidity and mortality of a lethal challenge with homologous A/Goose/Anhui/08, but only 80% of chickens were protected after challenge with heterologous A/Goose/Guangdong/1/96. The N3 neuraminidase marker distinguishes rH5N3-DL-vaccinated from H5N1-infected animals. rH5N3-DL is thus a promising vaccine candidate to combat highly pathogenic avian influenza virus infections. The A/Goose/Dalian/3/01 virus could be a promising candidate as providing internal genes donors with high-yield properties in reverse-genetics system and might be applicable for future avian influenza vaccine development.
http://www.sciencedirect.com/science/article/pii/S0378113512003902