tetano
Editor, Senior Moderator
Langzhou Song1*, Valerian Nakaar2, Uma Kavita1, Albert Price2, Jim Huleatt2?, Jie Tang2, Andrea Jacobs2, Ge Liu1, Yan Huang2, Priyanka Desai2, Gail Maksymiuk2, Virginia Takahashi2, Scott Umlauf1, Lucia Reiserova1, Rodney Bell1, Hong Li1, Yi Zhang1, William F. McDonald2, T. J. Powell2, Lynda Tussey1
1 VaxInnate Corporation, Cranbury, New Jersey, United States of America, 2 VaxInnate Corporation, New Haven, Connecticut, United States of America
Abstract Top
It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5 ligand, flagellin. These fusion proteins are efficiently expressed in standard E. coli fermentation systems and the HA moiety can be faithfully refolded to take on the native conformation of the globular head. In mouse models of influenza infection, the vaccines elicit robust antibody responses that mitigate disease and protect mice from lethal challenge. These immunologically potent vaccines can be efficiently manufactured to support pandemic response, pre-pandemic and seasonal vaccines.
Citation: Song L, Nakaar V, Kavita U, Price A, Huleatt J, et al. (2008) Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs. PLoS ONE 3(5): e2257. doi:10.1371/journal.pone.0002257
http://www.plosone.org/article/info...7;jsessionid=66F380CC028AA330385C54C3B25AE539
1 VaxInnate Corporation, Cranbury, New Jersey, United States of America, 2 VaxInnate Corporation, New Haven, Connecticut, United States of America
Abstract Top
It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5 ligand, flagellin. These fusion proteins are efficiently expressed in standard E. coli fermentation systems and the HA moiety can be faithfully refolded to take on the native conformation of the globular head. In mouse models of influenza infection, the vaccines elicit robust antibody responses that mitigate disease and protect mice from lethal challenge. These immunologically potent vaccines can be efficiently manufactured to support pandemic response, pre-pandemic and seasonal vaccines.
Citation: Song L, Nakaar V, Kavita U, Price A, Huleatt J, et al. (2008) Efficacious Recombinant Influenza Vaccines Produced by High Yield Bacterial Expression: A Solution to Global Pandemic and Seasonal Needs. PLoS ONE 3(5): e2257. doi:10.1371/journal.pone.0002257
http://www.plosone.org/article/info...7;jsessionid=66F380CC028AA330385C54C3B25AE539