tetano
Editor, Senior Moderator
Int Immunopharmacol. 2011 Oct 7. [Epub ahead of print]
Effects of oral administration of yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 and its exopolysaccharides against influenza virus infection in mice.
Nagai T, Makino S, Ikegami S, Itoh H, Yamada H.
Source
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan; Oriental Medicine Research Center, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Abstract
Yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (1073R-1) has been shown to reduce the risk of catching cold in the healthy elderly by two independent cohort studies (Makino et al., Br. J. Nutr., 104, 998-1006, 2010). In addition, the exopolysaccharides (EPS) produced by 1073R-1 were also reported to exert immunostimulatory effects in mice such as the augmentation of NK cell activity (Makino et al., J. Dairy Sci., 89, 2873-81, 2006). So, we investigated anti-influenza virus effects of this yogurt and EPS in mice. The yogurt (0.4ml/day) and EPS (20μg/day) were orally administered to BALB/c mice for 21days prior to intranasal infection with influenza virus A/PR/8/34 (H1N1). Post infection (p.i.), survival of the mice in each group was observed every day for 21days. As a result, the survival periods were prolonged in both yogurt- and EPS-treated groups compared to the water-treated group. Moreover, in these groups, we observed significant decrease of influenza virus titer and significant increase of anti-influenza virus antibodies (IgA, IgG(1)) in the bronchoalveolar lavage fluid (BALF) at 4days p.i. NK cell activity of splenocytes in both groups was also increased significantly compared to the water-treated groups. EPS was further fractionated into neutral EPS (NPS) and acidic EPS (APS), and the NPS (20μg/day) or the APS (20μg/day) was orally administered to mice for 21days prior to intranasal infection with influenza virus A/PR/8/34. The survival periods were prolonged in APS-treated group compared to the water-treated group, but not in NPS-treated group. Consequently, we concluded that the yogurt fermented with 1073R-1 exerted anti-influenza virus effects in mice by its immunopotentiating activity, and suggested that the APS produced by 1073R-1 was one of active ingredients.
Copyright ? 2011. Published by Elsevier B.V.
PMID:
21986509
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21986509
Effects of oral administration of yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 and its exopolysaccharides against influenza virus infection in mice.
Nagai T, Makino S, Ikegami S, Itoh H, Yamada H.
Source
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan; Oriental Medicine Research Center, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Abstract
Yogurt fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (1073R-1) has been shown to reduce the risk of catching cold in the healthy elderly by two independent cohort studies (Makino et al., Br. J. Nutr., 104, 998-1006, 2010). In addition, the exopolysaccharides (EPS) produced by 1073R-1 were also reported to exert immunostimulatory effects in mice such as the augmentation of NK cell activity (Makino et al., J. Dairy Sci., 89, 2873-81, 2006). So, we investigated anti-influenza virus effects of this yogurt and EPS in mice. The yogurt (0.4ml/day) and EPS (20μg/day) were orally administered to BALB/c mice for 21days prior to intranasal infection with influenza virus A/PR/8/34 (H1N1). Post infection (p.i.), survival of the mice in each group was observed every day for 21days. As a result, the survival periods were prolonged in both yogurt- and EPS-treated groups compared to the water-treated group. Moreover, in these groups, we observed significant decrease of influenza virus titer and significant increase of anti-influenza virus antibodies (IgA, IgG(1)) in the bronchoalveolar lavage fluid (BALF) at 4days p.i. NK cell activity of splenocytes in both groups was also increased significantly compared to the water-treated groups. EPS was further fractionated into neutral EPS (NPS) and acidic EPS (APS), and the NPS (20μg/day) or the APS (20μg/day) was orally administered to mice for 21days prior to intranasal infection with influenza virus A/PR/8/34. The survival periods were prolonged in APS-treated group compared to the water-treated group, but not in NPS-treated group. Consequently, we concluded that the yogurt fermented with 1073R-1 exerted anti-influenza virus effects in mice by its immunopotentiating activity, and suggested that the APS produced by 1073R-1 was one of active ingredients.
Copyright ? 2011. Published by Elsevier B.V.
PMID:
21986509
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21986509