tetano
Editor, Senior Moderator
J Control Release. 2017 Sep 7. pii: S0168-3659(17)30844-1. doi: 10.1016/j.jconrel.2017.09.010. [Epub ahead of print]
[h=1]Effect of zymosan and poly (I:C) adjuvants on responses to microneedle immunization coated with whole inactivated influenza vaccine.[/h] Shin JH[SUP]1[/SUP], Noh JY[SUP]2[/SUP], Kim KH[SUP]1[/SUP], Park JK[SUP]3[/SUP], Lee JH[SUP]2[/SUP], Jeong SD[SUP]1[/SUP], Jung DY[SUP]1[/SUP], Song CS[SUP]2[/SUP], Kim YC[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Microneedles are the micrometer size devices used for the delivery of vaccines and biotherapeutics. In order to increase the vaccine efficacy and reduce the antigen dose, there is a significant need to find some adjuvants for the microneedle vaccination. In this study, zymosan, which is the cell wall preparation of Saccharomyces cerevisiae, or poly (I:C) was coated on a microneedle with inactivated influenza virus, and then immunized into BALB/c mouse to determine the immunogenicity, protection and synergetic effect between two adjuvants. As a result, the group administered with zymosan and vaccine antigen showed significantly stronger IgG response, HI titer and IgG subtypes without any adverse effects, compared to the group immunized with the vaccine antigen alone. Also, there were enhanced cellular immune responses in the group received adjuvant with vaccine antigen. In addition, they showed superior protection and lung viral reduction against lethal viral challenge. Taken together, this study confirms that zymosan can be used as an immunostimulant for microneedle vaccination.
Copyright ? 2017. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Adjuvant; Influenza; Microneedle; Skin; Vaccine; Zymosan
PMID: 28890214 DOI: 10.1016/j.jconrel.2017.09.010
[h=1]Effect of zymosan and poly (I:C) adjuvants on responses to microneedle immunization coated with whole inactivated influenza vaccine.[/h] Shin JH[SUP]1[/SUP], Noh JY[SUP]2[/SUP], Kim KH[SUP]1[/SUP], Park JK[SUP]3[/SUP], Lee JH[SUP]2[/SUP], Jeong SD[SUP]1[/SUP], Jung DY[SUP]1[/SUP], Song CS[SUP]2[/SUP], Kim YC[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Microneedles are the micrometer size devices used for the delivery of vaccines and biotherapeutics. In order to increase the vaccine efficacy and reduce the antigen dose, there is a significant need to find some adjuvants for the microneedle vaccination. In this study, zymosan, which is the cell wall preparation of Saccharomyces cerevisiae, or poly (I:C) was coated on a microneedle with inactivated influenza virus, and then immunized into BALB/c mouse to determine the immunogenicity, protection and synergetic effect between two adjuvants. As a result, the group administered with zymosan and vaccine antigen showed significantly stronger IgG response, HI titer and IgG subtypes without any adverse effects, compared to the group immunized with the vaccine antigen alone. Also, there were enhanced cellular immune responses in the group received adjuvant with vaccine antigen. In addition, they showed superior protection and lung viral reduction against lethal viral challenge. Taken together, this study confirms that zymosan can be used as an immunostimulant for microneedle vaccination.
Copyright ? 2017. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Adjuvant; Influenza; Microneedle; Skin; Vaccine; Zymosan
PMID: 28890214 DOI: 10.1016/j.jconrel.2017.09.010