tetano
Editor, Senior Moderator
Vet Res Forum. 2019 Fall;10(4):293-297. doi: 10.30466/vrf.2018.75860.2013. Epub 2019 Dec 15.
Effect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H[SUB]9[/SUB]N[SUB]2[/SUB] in turkey by real time PCR.
Hashemzade F[SUP]1[/SUP], Mayahi M[SUP]2[/SUP], Shoshtary A[SUP]3[/SUP], Reza Seyfi Abad Shapouri M[SUP]4[/SUP], Ghorbanpoor M[SUP]4[/SUP].
Author information
Abstract
Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected with 10[SUP]4[/SUP] CID[SUB]50[/SUB] of IBDV via intra-bursal route on day 1; Groups 1 and 3 were each infected with 10[SUP]6[/SUP] EID50 of AIV (H[SUB]9[/SUB]N[SUB]2[/SUB]) via the oculo-nasal routes on day 30. All groups were vaccinated against Newcastle disease vaccine (NDV). Detection of avian influenza virus H[SUB]9[/SUB]N[SUB]2[/SUB] in trachea and cloaca swabs and in the tissues, was confirmed by Real-time polymerase chain reaction. Anti- NDV-AIV and anti-IBD titers were measured using HI and ELISA tests, respectively. The present study showed that infectious bursal disease changed the pathogenesis of (AIV) H[SUB]9[/SUB]N[SUB]2[/SUB] by affecting AI virus replication and resulted in an increase shedding due to prolonged duration of sever clinical signs. The extent of shedding and virus replication need further study.
? 2019 Urmia University. All rights reserved.
KEYWORDS:
Avian influenza; Infectious bursal disease virus; Real time PCR; Turkey
PMID:32206224PMCID:PMC7065577DOI:10.30466/vrf.2018.75860.2013
Effect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H[SUB]9[/SUB]N[SUB]2[/SUB] in turkey by real time PCR.
Hashemzade F[SUP]1[/SUP], Mayahi M[SUP]2[/SUP], Shoshtary A[SUP]3[/SUP], Reza Seyfi Abad Shapouri M[SUP]4[/SUP], Ghorbanpoor M[SUP]4[/SUP].
Author information
Abstract
Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected with 10[SUP]4[/SUP] CID[SUB]50[/SUB] of IBDV via intra-bursal route on day 1; Groups 1 and 3 were each infected with 10[SUP]6[/SUP] EID50 of AIV (H[SUB]9[/SUB]N[SUB]2[/SUB]) via the oculo-nasal routes on day 30. All groups were vaccinated against Newcastle disease vaccine (NDV). Detection of avian influenza virus H[SUB]9[/SUB]N[SUB]2[/SUB] in trachea and cloaca swabs and in the tissues, was confirmed by Real-time polymerase chain reaction. Anti- NDV-AIV and anti-IBD titers were measured using HI and ELISA tests, respectively. The present study showed that infectious bursal disease changed the pathogenesis of (AIV) H[SUB]9[/SUB]N[SUB]2[/SUB] by affecting AI virus replication and resulted in an increase shedding due to prolonged duration of sever clinical signs. The extent of shedding and virus replication need further study.
? 2019 Urmia University. All rights reserved.
KEYWORDS:
Avian influenza; Infectious bursal disease virus; Real time PCR; Turkey
PMID:32206224PMCID:PMC7065577DOI:10.30466/vrf.2018.75860.2013