tetano
Editor, Senior Moderator
Talanta. 2016 Aug 1;155:250-7. doi: 10.1016/j.talanta.2016.04.047. Epub 2016 Apr 25.
[h=1]Dual immunosensor based on methylene blue-electroadsorbed graphene oxide for rapid detection of the influenza A virus antigen.[/h] Veerapandian M[SUP]1[/SUP], Hunter R[SUP]1[/SUP], Neethirajan S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid detection of influenza viral infections in poultry facilities is advantageous in several aspects such as environmental/personal safety, food-security, and socio-economy. Herein, we report the development of an electrochemical-based dual-sensor platform composed of methylene blue-electroadsorbed graphene oxide nanostructures modified with monoclonal antibodies against the HA proteins of H5N1 and H1N1. Bio-functional layers comprised of chitosan and protein-A molecules were implemented at the interface of the sensor element and antibodies, which synergistically enriched the bio-activity of immobilized antibodies for the immune complex formation. The differential pulse voltammetric signals resulted from the developed immunosensor platform exhibited a good correlation (R(2)=0.9978 for H1N1 and R(2)=0.9997 for H5N1) for the wide range of target concentrations 25-500pM). Chronoamperometric study also revealed the amplified current sensitivity of the immunoelectrodes even at the picomolar level. The proposed immunosensor design not only provides rapid analytical response time (<1min) but simplicity in fabrication and instrumentation, which paves an attractive platform for on-farm monitoring of viral infections.
Copyright ? 2016 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Dual immunosensor; Electroadsorption; Graphene oxide; H1N1 flu detection; H5N1 flu detection; Methylene blue
PMID: 27216681 [PubMed - in process]
[h=1]Dual immunosensor based on methylene blue-electroadsorbed graphene oxide for rapid detection of the influenza A virus antigen.[/h] Veerapandian M[SUP]1[/SUP], Hunter R[SUP]1[/SUP], Neethirajan S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid detection of influenza viral infections in poultry facilities is advantageous in several aspects such as environmental/personal safety, food-security, and socio-economy. Herein, we report the development of an electrochemical-based dual-sensor platform composed of methylene blue-electroadsorbed graphene oxide nanostructures modified with monoclonal antibodies against the HA proteins of H5N1 and H1N1. Bio-functional layers comprised of chitosan and protein-A molecules were implemented at the interface of the sensor element and antibodies, which synergistically enriched the bio-activity of immobilized antibodies for the immune complex formation. The differential pulse voltammetric signals resulted from the developed immunosensor platform exhibited a good correlation (R(2)=0.9978 for H1N1 and R(2)=0.9997 for H5N1) for the wide range of target concentrations 25-500pM). Chronoamperometric study also revealed the amplified current sensitivity of the immunoelectrodes even at the picomolar level. The proposed immunosensor design not only provides rapid analytical response time (<1min) but simplicity in fabrication and instrumentation, which paves an attractive platform for on-farm monitoring of viral infections.
Copyright ? 2016 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Dual immunosensor; Electroadsorption; Graphene oxide; H1N1 flu detection; H5N1 flu detection; Methylene blue
PMID: 27216681 [PubMed - in process]