tetano
Editor, Senior Moderator
Dokl Biochem Biophys
. 2021 Dec 4.
doi: 10.1134/S160767292201001X. Online ahead of print.
Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
V G Krut[SUP] 1 [/SUP], I V Astrakhantseva[SUP] 1 [/SUP], S A Chuvpilo[SUP] 1 [/SUP], G A Efimov[SUP] 2 [/SUP], S G Ambaryan[SUP] 1 [/SUP], M S Drutskaya[SUP] 1 3 [/SUP], S A Nedospasov[SUP] 4 5 [/SUP]
Affiliations
Abstract
SARS-CoV-2 is a new coronavirus that is the cause of COVID-19 pandemic. To enter the cell, the virus interacts via its surface S protein with angiotensin-converting enzyme 2 (ACE2), the main entry receptor on the cell membrane. Most of protective antibodies, including those induced by vaccinations, target the S protein, preventing its interaction with the ACE2 receptor. We have evaluated an alternative strategy for blocking the S-ACE2 interaction using new antipeptide antibodies to the N-terminus of the ACE2 molecule. These antibodies allow detection of human ACE2 in vitro and ex vivo.
Keywords: COVID-19; peplomers; peptides; receptor.
. 2021 Dec 4.
doi: 10.1134/S160767292201001X. Online ahead of print.
Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S Protein
V G Krut[SUP] 1 [/SUP], I V Astrakhantseva[SUP] 1 [/SUP], S A Chuvpilo[SUP] 1 [/SUP], G A Efimov[SUP] 2 [/SUP], S G Ambaryan[SUP] 1 [/SUP], M S Drutskaya[SUP] 1 3 [/SUP], S A Nedospasov[SUP] 4 5 [/SUP]
Affiliations
- PMID: 34874527
- DOI: 10.1134/S160767292201001X
Abstract
SARS-CoV-2 is a new coronavirus that is the cause of COVID-19 pandemic. To enter the cell, the virus interacts via its surface S protein with angiotensin-converting enzyme 2 (ACE2), the main entry receptor on the cell membrane. Most of protective antibodies, including those induced by vaccinations, target the S protein, preventing its interaction with the ACE2 receptor. We have evaluated an alternative strategy for blocking the S-ACE2 interaction using new antipeptide antibodies to the N-terminus of the ACE2 molecule. These antibodies allow detection of human ACE2 in vitro and ex vivo.
Keywords: COVID-19; peplomers; peptides; receptor.