tetano
Editor, Senior Moderator
Antiviral Res. 2017 May 23. pii: S0166-3542(17)30009-8. doi: 10.1016/j.antiviral.2017.05.009. [Epub ahead of print]
[h=1]Distinct patterns of cellular immune response elicited by influenza non-adjuvanted and AS03-adjuvanted monovalent H1N1(pdm09) vaccine.[/h] Giarola-Silva S[SUP]1[/SUP], Coelho-Dos-Reis JGA[SUP]1[/SUP], Mour?o MM[SUP]2[/SUP], Campi-Azevedo AC[SUP]1[/SUP], Nakagaki Silva EE[SUP]2[/SUP], Luiza-Silva M[SUP]3[/SUP], Martins MA[SUP]1[/SUP], de Oliveira Silveira Cassette AC[SUP]1[/SUP], Batista MA[SUP]1[/SUP], Peruhype-Magalh?es V[SUP]1[/SUP], do Valle Antonelli LR[SUP]4[/SUP], Leite Ribeiro JG[SUP]5[/SUP], El?i-Santos SM[SUP]6[/SUP], Machado AV[SUP]7[/SUP], Teixeira-Carvalho A[SUP]1[/SUP], Martins-Filho OA[SUP]1[/SUP], Ara?jo MSS[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The study aimed at identifying biomarkers of immune response elicited by non-adjuvanted-(NAV) and adjuvanted-(AV) H1N1(pdm09) vaccines. The results showed that despite both vaccines elicited similar levels of anti-H1N1 antibodies at day30 after vaccination, higher reactivity was observed in AV at day180. While AV induced early changes in cell-surface molecules on monocytes, CD4[SUP]+[/SUP], CD8[SUP]+[/SUP] T-cells and B-cells, NAV triggered minor changes, starting later on at day3. Furthermore, AV induced a late and persistent increase in TLR gene expression after day3, except for tlr4, while NAV displayed earlier but transient tlr3/4/7/9 up-regulation. Contrasting with NAV, prominent chemokine gene expression (cxcl8,cxcl9,ccl5) and a broad spectrum up-regulation of plasmatic biomarkers (CXCL8,IL-6,IL-1β,IL-12,IL-10) was evident in AV, which showed a major involvement of TNF and IL-10. Similarly, AV induced a robust IL-10-modulated proinflammatory storm, with early and persistent involvement of TNF-α/IL-12/IFN-γ axis derived from NK-cells, CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cells along with promiscuous production of IL-4/IL-5/IL-13. Conversely, NAV promotes a concise and restricted intracytoplasmic chemokine/cytokine response, essentially mediated by TNF-α and IL-4, with late IL-10 production by CD8[SUP]+[/SUP] T-cells. Systems biology approach underscored that AV guided the formation of an imbricate network characterized by a progressive increase in the number of neighborhood connections amongst innate and adaptive immunity. In AV, the early cross-talk between innate and adaptive immunity, followed by the triad NK/CD4[SUP]+[/SUP]/CD8[SUP]+[/SUP] T-cells at day3, sponsored a later/robust biomarker network. These findings indicate the relevance of adjuvanted vaccination to orchestrate broad, balanced and multifactorial cellular immune events that lead ultimately to a stronger H1N1 humoral immunity.
Copyright ? 2017. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] AS03 adjuvant; Biomarker signature; H1N1 vaccine; Immune response
PMID: 28549970 DOI: 10.1016/j.antiviral.2017.05.009
[h=1]Distinct patterns of cellular immune response elicited by influenza non-adjuvanted and AS03-adjuvanted monovalent H1N1(pdm09) vaccine.[/h] Giarola-Silva S[SUP]1[/SUP], Coelho-Dos-Reis JGA[SUP]1[/SUP], Mour?o MM[SUP]2[/SUP], Campi-Azevedo AC[SUP]1[/SUP], Nakagaki Silva EE[SUP]2[/SUP], Luiza-Silva M[SUP]3[/SUP], Martins MA[SUP]1[/SUP], de Oliveira Silveira Cassette AC[SUP]1[/SUP], Batista MA[SUP]1[/SUP], Peruhype-Magalh?es V[SUP]1[/SUP], do Valle Antonelli LR[SUP]4[/SUP], Leite Ribeiro JG[SUP]5[/SUP], El?i-Santos SM[SUP]6[/SUP], Machado AV[SUP]7[/SUP], Teixeira-Carvalho A[SUP]1[/SUP], Martins-Filho OA[SUP]1[/SUP], Ara?jo MSS[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The study aimed at identifying biomarkers of immune response elicited by non-adjuvanted-(NAV) and adjuvanted-(AV) H1N1(pdm09) vaccines. The results showed that despite both vaccines elicited similar levels of anti-H1N1 antibodies at day30 after vaccination, higher reactivity was observed in AV at day180. While AV induced early changes in cell-surface molecules on monocytes, CD4[SUP]+[/SUP], CD8[SUP]+[/SUP] T-cells and B-cells, NAV triggered minor changes, starting later on at day3. Furthermore, AV induced a late and persistent increase in TLR gene expression after day3, except for tlr4, while NAV displayed earlier but transient tlr3/4/7/9 up-regulation. Contrasting with NAV, prominent chemokine gene expression (cxcl8,cxcl9,ccl5) and a broad spectrum up-regulation of plasmatic biomarkers (CXCL8,IL-6,IL-1β,IL-12,IL-10) was evident in AV, which showed a major involvement of TNF and IL-10. Similarly, AV induced a robust IL-10-modulated proinflammatory storm, with early and persistent involvement of TNF-α/IL-12/IFN-γ axis derived from NK-cells, CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cells along with promiscuous production of IL-4/IL-5/IL-13. Conversely, NAV promotes a concise and restricted intracytoplasmic chemokine/cytokine response, essentially mediated by TNF-α and IL-4, with late IL-10 production by CD8[SUP]+[/SUP] T-cells. Systems biology approach underscored that AV guided the formation of an imbricate network characterized by a progressive increase in the number of neighborhood connections amongst innate and adaptive immunity. In AV, the early cross-talk between innate and adaptive immunity, followed by the triad NK/CD4[SUP]+[/SUP]/CD8[SUP]+[/SUP] T-cells at day3, sponsored a later/robust biomarker network. These findings indicate the relevance of adjuvanted vaccination to orchestrate broad, balanced and multifactorial cellular immune events that lead ultimately to a stronger H1N1 humoral immunity.
Copyright ? 2017. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] AS03 adjuvant; Biomarker signature; H1N1 vaccine; Immune response
PMID: 28549970 DOI: 10.1016/j.antiviral.2017.05.009