tetano
Editor, Senior Moderator
Immunology. 2014 Mar 20. doi: 10.1111/imm.12287. [Epub ahead of print]
Distinct B cell populations contribute to vaccine antigen specific-antibody production in a transgenic mouse model.
O E1, Ko EJ, Kim MC, Lee YT, Song JM, Kwon YM, Compans RW, Kang SM.
Author information
Abstract
The generation of memory B cells by vaccination plays a critical role in maintaining antigen specific antibodies and producing antibody responses upon re-exposure to a pathogen. B cell populations contributing to antibody production and protection by vaccination remain poorly defined. We used influenza virus-like particle (VLP) vaccine in a transgenic mouse model that would identify germinal center-derived memory B cells with the expression of yellow fluorescent protein (YFP+ cells). Immunization with influenza VLP vaccine did not induce significant increases in YFP+ cells although vaccine antigen-specific antibodies in sera were found to confer protection against a lethal dose of influenza A virus (A/PR8). In addition, CD43+ B220- populations with low YFP+ cells mainly contributed to the production of vaccine antigen-specific IgG isotype-switched antibodies whereas CD43- B220+ populations with high YFP+ cells were able to produce vaccine antigen-specific IgM antibodies. Challenge infection of immunized transgenic mice with live influenza A virus resulted in significant increases in YFP+ cells in the B220- populations of spleen and bone marrow cells. These results suggest that CD43+ B220- B cells generated by vaccination are important for producing influenza vaccine antigen-specific antibodies and conferring protection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza virus, Memory B cells, Plasma cells, Protection, Vaccine
PMID:
24645831
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24645831
Distinct B cell populations contribute to vaccine antigen specific-antibody production in a transgenic mouse model.
O E1, Ko EJ, Kim MC, Lee YT, Song JM, Kwon YM, Compans RW, Kang SM.
Author information
Abstract
The generation of memory B cells by vaccination plays a critical role in maintaining antigen specific antibodies and producing antibody responses upon re-exposure to a pathogen. B cell populations contributing to antibody production and protection by vaccination remain poorly defined. We used influenza virus-like particle (VLP) vaccine in a transgenic mouse model that would identify germinal center-derived memory B cells with the expression of yellow fluorescent protein (YFP+ cells). Immunization with influenza VLP vaccine did not induce significant increases in YFP+ cells although vaccine antigen-specific antibodies in sera were found to confer protection against a lethal dose of influenza A virus (A/PR8). In addition, CD43+ B220- populations with low YFP+ cells mainly contributed to the production of vaccine antigen-specific IgG isotype-switched antibodies whereas CD43- B220+ populations with high YFP+ cells were able to produce vaccine antigen-specific IgM antibodies. Challenge infection of immunized transgenic mice with live influenza A virus resulted in significant increases in YFP+ cells in the B220- populations of spleen and bone marrow cells. These results suggest that CD43+ B220- B cells generated by vaccination are important for producing influenza vaccine antigen-specific antibodies and conferring protection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Influenza virus, Memory B cells, Plasma cells, Protection, Vaccine
PMID:
24645831
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24645831