tetano
Editor, Senior Moderator
Biotechnol Bioeng. 2015 May 5. doi: 10.1002/bit.25634. [Epub ahead of print]
[h=1]Dissolved carbon dioxide determines the productivity of a recombinant hemagglutinin component of an influenza vaccine produced by insect cells.[/h] Meghrous J[SUP]1[/SUP], Khramtsov N, Buckland BC, Cox MM, Palomares LA, Srivastava IK.
[h=3]Author information[/h]
[h=3]Abstract[/h] Dissolved carbon dioxide (dCO[SUB]2[/SUB] ) accumulation during cell culture has been recognized as an important parameter that needs to be controlled for successful scale-up of animal cell culture because above a certain concentration there are adverse effects on cell growth performance and protein production. We investigated the effect of accumulation of dCO[SUB]2[/SUB] in bioreactor cultures of expresSF + ? insect cells infected with recombinant baculoviruses expressing recombinant influenza virus hemagglutinins (rHA). Different strategies for bioreactor cultures were used to obtain various ranges of concentrations of dCO[SUB]2[/SUB] (<50, 50-100, 100-200, and >200 mmHg) and to determine their effects on recombinant protein production and cell metabolic activity. We show that the accumulation of dCO[SUB]2[/SUB] at levels > 100 mmHg resulted in reduced metabolic activity, slowed cell growth, prolonged culture viability after infection, and decreased infection kinetics. The reduced rHA yields were not caused by the decrease in the extracellular pH that resulted from dCO[SUB]2[/SUB] accumulation, but were most likely due to the effect of dCO[SUB]2[/SUB] accumulation in cells. The results obtained here at the 2 liter scale have been used for the design of large-scale processes to manufacture the rHA based recombinant vaccine Flublok? at the 2500 liter scale. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Flublok; baculovirus; dissolved CO2; hemagglutinin; influenza vaccine; scale-up
PMID: 25943562 [PubMed - as supplied by publisher]
[h=1]Dissolved carbon dioxide determines the productivity of a recombinant hemagglutinin component of an influenza vaccine produced by insect cells.[/h] Meghrous J[SUP]1[/SUP], Khramtsov N, Buckland BC, Cox MM, Palomares LA, Srivastava IK.
[h=3]Author information[/h]
[h=3]Abstract[/h] Dissolved carbon dioxide (dCO[SUB]2[/SUB] ) accumulation during cell culture has been recognized as an important parameter that needs to be controlled for successful scale-up of animal cell culture because above a certain concentration there are adverse effects on cell growth performance and protein production. We investigated the effect of accumulation of dCO[SUB]2[/SUB] in bioreactor cultures of expresSF + ? insect cells infected with recombinant baculoviruses expressing recombinant influenza virus hemagglutinins (rHA). Different strategies for bioreactor cultures were used to obtain various ranges of concentrations of dCO[SUB]2[/SUB] (<50, 50-100, 100-200, and >200 mmHg) and to determine their effects on recombinant protein production and cell metabolic activity. We show that the accumulation of dCO[SUB]2[/SUB] at levels > 100 mmHg resulted in reduced metabolic activity, slowed cell growth, prolonged culture viability after infection, and decreased infection kinetics. The reduced rHA yields were not caused by the decrease in the extracellular pH that resulted from dCO[SUB]2[/SUB] accumulation, but were most likely due to the effect of dCO[SUB]2[/SUB] accumulation in cells. The results obtained here at the 2 liter scale have been used for the design of large-scale processes to manufacture the rHA based recombinant vaccine Flublok? at the 2500 liter scale. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Flublok; baculovirus; dissolved CO2; hemagglutinin; influenza vaccine; scale-up
PMID: 25943562 [PubMed - as supplied by publisher]