tetano
Editor, Senior Moderator
Dis Model Mech
. 2022 Oct 12;dmm.049632.
doi: 10.1242/dmm.049632. Online ahead of print.
Comparison of the pathogenesis of SARS-CoV-2 infection in K18-hACE2 mice and Syrian golden hamster models
Haengdueng Jeong[SUP] 1 [/SUP], Youn Woo Lee[SUP] 2 [/SUP], In Ho Park[SUP] 1 3 [/SUP], Hyuna Noh[SUP] 4 [/SUP], Sung-Hee Kim[SUP] 1 [/SUP], Jiseon Kim[SUP] 1 [/SUP], Donghun Jeon[SUP] 1 [/SUP], Hui Jeong Jang[SUP] 2 [/SUP], Jooyeon Oh[SUP] 5 [/SUP], Dain On[SUP] 4 6 [/SUP], Chanyang Uhm[SUP] 1 [/SUP], Kyungrae Cho[SUP] 1 [/SUP], Heeju Oh[SUP] 1 [/SUP], Suhyeon Yoon[SUP] 4 [/SUP], Jung Seon Seo[SUP] 1 [/SUP], Jeong Jin Kim[SUP] 1 [/SUP], Sang-Hyuk Seok[SUP] 7 [/SUP], Yu Jin Lee[SUP] 7 [/SUP], Seung-Min Hong[SUP] 8 [/SUP], Se-Hee An[SUP] 8 [/SUP], Seo Yeon Kim[SUP] 9 [/SUP], Young Been Kim[SUP] 9 [/SUP], Ji-Yeon Hwang[SUP] 9 [/SUP], Hyo-Jung Lee[SUP] 10 [/SUP], Hong Bin Kim[SUP] 11 [/SUP], Dae Gwin Jeong[SUP] 12 [/SUP], Daesub Song[SUP] 13 [/SUP], Manki Song[SUP] 14 [/SUP], Man-Seong Park[SUP] 15 [/SUP], Kang-Seuk Choi[SUP] 8 [/SUP], Jun Won Park[SUP] 7 [/SUP], Jun-Young Seo[SUP] 1 [/SUP], Jun-Won Yun[SUP] 16 [/SUP], Jeon-Soo Shin[SUP] 1 3 5 [/SUP], Ho-Young Lee[SUP] 2 17 [/SUP], Ki Taek Nam[SUP] 1 [/SUP], Je Kyung Seong[SUP] 4 6 18 19 [/SUP]
Affiliations
Abstract
SARS-CoV-2, the etiological agent of COVID-19, causes life-threatening disease. This novel coronavirus enters host cells via the respiratory tract, promoting the formation of severe pulmonary lesions and systemic disease. Few animal models can simulate the clinical signs and pathology of COVID-19 patients. Diverse preclinical studies using K18-hACE2 mice and Syrian golden hamsters, which are highly permissive to SARS-CoV-2 in the respiratory tract, are emerging; however, the systemic pathogenesis and cellular tropism of these models remain obscure. We intranasally infected K18-hACE2 mice and Syrian golden hamsters with SARS-CoV-2, and compared the clinical features, pathogenesis, cellular tropism, and infiltrated immune-cell subsets. In K18-hACE2 mice, SARS-CoV-2 persistently replicated in alveolar cells and caused pulmonary and extra-pulmonary disease, resulting in fatal outcomes. Conversely, in Syrian golden hamsters, transient SARS-CoV-2 infection in bronchial cells caused reversible pulmonary disease, without mortality. Our findings provide comprehensive insights into the pathogenic spectrum of COVID-19 using pre-clinical models.
Keywords: Covid-19; K18-hACE2 mice; Pathogenesis; SARS-CoV-2; Syrian golden hamster.
. 2022 Oct 12;dmm.049632.
doi: 10.1242/dmm.049632. Online ahead of print.
Comparison of the pathogenesis of SARS-CoV-2 infection in K18-hACE2 mice and Syrian golden hamster models
Haengdueng Jeong[SUP] 1 [/SUP], Youn Woo Lee[SUP] 2 [/SUP], In Ho Park[SUP] 1 3 [/SUP], Hyuna Noh[SUP] 4 [/SUP], Sung-Hee Kim[SUP] 1 [/SUP], Jiseon Kim[SUP] 1 [/SUP], Donghun Jeon[SUP] 1 [/SUP], Hui Jeong Jang[SUP] 2 [/SUP], Jooyeon Oh[SUP] 5 [/SUP], Dain On[SUP] 4 6 [/SUP], Chanyang Uhm[SUP] 1 [/SUP], Kyungrae Cho[SUP] 1 [/SUP], Heeju Oh[SUP] 1 [/SUP], Suhyeon Yoon[SUP] 4 [/SUP], Jung Seon Seo[SUP] 1 [/SUP], Jeong Jin Kim[SUP] 1 [/SUP], Sang-Hyuk Seok[SUP] 7 [/SUP], Yu Jin Lee[SUP] 7 [/SUP], Seung-Min Hong[SUP] 8 [/SUP], Se-Hee An[SUP] 8 [/SUP], Seo Yeon Kim[SUP] 9 [/SUP], Young Been Kim[SUP] 9 [/SUP], Ji-Yeon Hwang[SUP] 9 [/SUP], Hyo-Jung Lee[SUP] 10 [/SUP], Hong Bin Kim[SUP] 11 [/SUP], Dae Gwin Jeong[SUP] 12 [/SUP], Daesub Song[SUP] 13 [/SUP], Manki Song[SUP] 14 [/SUP], Man-Seong Park[SUP] 15 [/SUP], Kang-Seuk Choi[SUP] 8 [/SUP], Jun Won Park[SUP] 7 [/SUP], Jun-Young Seo[SUP] 1 [/SUP], Jun-Won Yun[SUP] 16 [/SUP], Jeon-Soo Shin[SUP] 1 3 5 [/SUP], Ho-Young Lee[SUP] 2 17 [/SUP], Ki Taek Nam[SUP] 1 [/SUP], Je Kyung Seong[SUP] 4 6 18 19 [/SUP]
Affiliations
- PMID: 36222118
- DOI: 10.1242/dmm.049632
Abstract
SARS-CoV-2, the etiological agent of COVID-19, causes life-threatening disease. This novel coronavirus enters host cells via the respiratory tract, promoting the formation of severe pulmonary lesions and systemic disease. Few animal models can simulate the clinical signs and pathology of COVID-19 patients. Diverse preclinical studies using K18-hACE2 mice and Syrian golden hamsters, which are highly permissive to SARS-CoV-2 in the respiratory tract, are emerging; however, the systemic pathogenesis and cellular tropism of these models remain obscure. We intranasally infected K18-hACE2 mice and Syrian golden hamsters with SARS-CoV-2, and compared the clinical features, pathogenesis, cellular tropism, and infiltrated immune-cell subsets. In K18-hACE2 mice, SARS-CoV-2 persistently replicated in alveolar cells and caused pulmonary and extra-pulmonary disease, resulting in fatal outcomes. Conversely, in Syrian golden hamsters, transient SARS-CoV-2 infection in bronchial cells caused reversible pulmonary disease, without mortality. Our findings provide comprehensive insights into the pathogenic spectrum of COVID-19 using pre-clinical models.
Keywords: Covid-19; K18-hACE2 mice; Pathogenesis; SARS-CoV-2; Syrian golden hamster.