tetano
Editor, Senior Moderator
CS Appl Mater Interfaces. 2019 Jan 28. doi: 10.1021/acsami.8b18898. [Epub ahead of print] [h=1]Digital Single Virus Immunoassay for Ultrasensitive Multiplex Avian Influenza Virus Detection Based on Fluorescent Magnetic Multifunctional Nanospheres.[/h] Wu Z, Zeng T, Guo WJ, Bai YY, Pang DW, Zhang ZL.
[h=3]Abstract[/h] Fluorescence method has made great progress in the construction of sensitive sensors, but the background fluorescence of matrix and photobleaching limit its broad application in clinical diagnosis. Here, we propose a digital single virus immunoassay for multiplex virus detection by using fluorescent magnetic multifunctional nanospheres as both capture carriers and signal labels. The superparamagnetism and strong magnetic response ability of nanospheres can realize efficient capture and separation of targets without sample pretreatment. Due to its distinguishable fluorescence imaging and photostability, the nanospheres enable single-particle counting for ultrasensitive multiplexed detection. Furthermore, the integration of digital analysis provided a reliable quantitative strategy for rare targets detection. Based on multifunctional nanospheres and digital analysis, a digital single virus immunoassay was proposed for simultaneous detection of H9N2, H1N1 and H7N9 avian influenza virus (AIV) without complex signal amplification, whose detection limits were 0.02 pg/mL. Owing to its good specificity and anti-interference ability, the method showed great potential in single biomolecules, multiplexed detection and early diagnosis of diseases.
PMID: 30688060 DOI: 10.1021/acsami.8b18898
[h=3]Abstract[/h] Fluorescence method has made great progress in the construction of sensitive sensors, but the background fluorescence of matrix and photobleaching limit its broad application in clinical diagnosis. Here, we propose a digital single virus immunoassay for multiplex virus detection by using fluorescent magnetic multifunctional nanospheres as both capture carriers and signal labels. The superparamagnetism and strong magnetic response ability of nanospheres can realize efficient capture and separation of targets without sample pretreatment. Due to its distinguishable fluorescence imaging and photostability, the nanospheres enable single-particle counting for ultrasensitive multiplexed detection. Furthermore, the integration of digital analysis provided a reliable quantitative strategy for rare targets detection. Based on multifunctional nanospheres and digital analysis, a digital single virus immunoassay was proposed for simultaneous detection of H9N2, H1N1 and H7N9 avian influenza virus (AIV) without complex signal amplification, whose detection limits were 0.02 pg/mL. Owing to its good specificity and anti-interference ability, the method showed great potential in single biomolecules, multiplexed detection and early diagnosis of diseases.
PMID: 30688060 DOI: 10.1021/acsami.8b18898