tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2011 Mar 16. [Epub ahead of print]
Differences in the antibody response between individuals with natural infection of and vaccination against pandemic H1N1 2009 influenza.
Chan KH, To KK, Hung IF, Zhang AJ, Chan JF, Cheng VC, Tse H, Che XY, Chen H, Yuen KY.
Department of Microbiology, Research Centre of Infection and Immunology, State Key Laboratory for Emerging Infectious Diseases, University of Hong Kong, Hong Kong Special Administrative Region, Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Abstract
The differential antibody response measured by the commonly used hemagglutination inhibition (HI) and microneutralization (MN) assays in patients with natural infection and vaccination has not been fully assessed. HI and conventional MN (CMN) assays were performed on sera from 651 patients with natural infection by pandemic H1N1 2009 influenza virus and 567 recipients of the corresponding vaccine. Surprisingly the overall seroprotection rate in vaccine recipients was only 44.8% and 35.1% by CMN and HI assay respectively. Antibody titers measured by CMN was significantly higher than that by HI in vaccine recipients aged ≥50 years, but these titers were not significantly different among those younger vaccine recipients. In contrast, HI titer was greater than CMN titer for the age group 16-29, but not significantly different in other age groups for natural infection. Lower antibody levels were found in both naturally infected patients and immunized recipients in the older than the younger age groups, but naturally infected patients exhibited higher HI and CMN titers than those vaccine recipients. In addition, we developed a rapid fluorescent foci microneutralization (FFMN) assay to test sera from those naturally infected patients. FFMN has a better correlation with CMN than HI (ρ=0.810 vs 0.684), which is expected of neutralizing antibody mainly targeted towards the inhibition of viral entry into cells. The higher antibody level elicited by natural infection than vaccination may be related to difference between antigen presentation by intramuscular route of vaccination and mucosal viral replication in mucosal cells of the respiratory tract.
PMID: 21411604 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21411604
Differences in the antibody response between individuals with natural infection of and vaccination against pandemic H1N1 2009 influenza.
Chan KH, To KK, Hung IF, Zhang AJ, Chan JF, Cheng VC, Tse H, Che XY, Chen H, Yuen KY.
Department of Microbiology, Research Centre of Infection and Immunology, State Key Laboratory for Emerging Infectious Diseases, University of Hong Kong, Hong Kong Special Administrative Region, Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Abstract
The differential antibody response measured by the commonly used hemagglutination inhibition (HI) and microneutralization (MN) assays in patients with natural infection and vaccination has not been fully assessed. HI and conventional MN (CMN) assays were performed on sera from 651 patients with natural infection by pandemic H1N1 2009 influenza virus and 567 recipients of the corresponding vaccine. Surprisingly the overall seroprotection rate in vaccine recipients was only 44.8% and 35.1% by CMN and HI assay respectively. Antibody titers measured by CMN was significantly higher than that by HI in vaccine recipients aged ≥50 years, but these titers were not significantly different among those younger vaccine recipients. In contrast, HI titer was greater than CMN titer for the age group 16-29, but not significantly different in other age groups for natural infection. Lower antibody levels were found in both naturally infected patients and immunized recipients in the older than the younger age groups, but naturally infected patients exhibited higher HI and CMN titers than those vaccine recipients. In addition, we developed a rapid fluorescent foci microneutralization (FFMN) assay to test sera from those naturally infected patients. FFMN has a better correlation with CMN than HI (ρ=0.810 vs 0.684), which is expected of neutralizing antibody mainly targeted towards the inhibition of viral entry into cells. The higher antibody level elicited by natural infection than vaccination may be related to difference between antigen presentation by intramuscular route of vaccination and mucosal viral replication in mucosal cells of the respiratory tract.
PMID: 21411604 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21411604