tetano
Editor, Senior Moderator
Diagnostics (Basel)
. 2021 Jun 22;11(7):1135.
doi: 10.3390/diagnostics11071135.
Evaluation of Anti-SARS-Cov-2 S-RBD IgG Antibodies after COVID-19 mRNA BNT162b2 Vaccine
Bruna Lo Sasso[SUP] 1 2 [/SUP], Rosaria Vincenza Giglio[SUP] 1 [/SUP], Matteo Vidali[SUP] 3 [/SUP], Concetta Scazzone[SUP] 1 [/SUP], Giulia Bivona[SUP] 1 [/SUP], Caterina Maria Gambino[SUP] 1 2 [/SUP], Anna Maria Ciaccio[SUP] 4 [/SUP], Luisa Agnello[SUP] 1 [/SUP], Marcello Ciaccio[SUP] 1 2 [/SUP]
Affiliations
Abstract
(1) Background: The evaluation of anti-spike protein receptor-binding domain (S-RBD) antibodies represents a useful tool to estimate the individual protection against Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infection; (2) Methods: We evaluated anti S-RBD IgG levels by indirect chemiluminescence immunoassay on Maglumi 800 (SNIBE, California) in 2248 vaccinated subjects without previous SARS-CoV-2 infection, 91 vaccinated individuals recovered from COVID-19, and 268 individuals recovered from COVID-19 who had not been vaccinated. Among those who were healthy and vaccinated, 352 subjects performed a re-dosing after about 72 days from the first measurement. (3) Results: Anti S-RBD IgG levels were lower in subjects with previous infection than vaccinated subjects, with or without previous infection (p < 0.001). No difference was observed between vaccinated subjects, with and without previous SARS-CoV-2 infection. Overall, anti-RBD IgG levels were higher in females than males (2110 vs. 1341 BAU/mL; p < 0.001) as well as in subjects with symptoms after vaccination than asymptomatic ones (2085 vs. 1332 BAU/mL; p = 0.001) and lower in older than younger subjects. Finally, a significant decrease in anti-RBD IgG levels was observed within a short period from a complete two-dose cycle vaccination. (4) Conclusions: Our results show an efficacy antibody response after vaccination with age-, time- and sex-related differences.
Keywords: COVID-19; S-RBD IgG; SARS-CoV-2; antibodies; immunosurvelliance; kinetic; spike; vaccination; vaccine.
. 2021 Jun 22;11(7):1135.
doi: 10.3390/diagnostics11071135.
Evaluation of Anti-SARS-Cov-2 S-RBD IgG Antibodies after COVID-19 mRNA BNT162b2 Vaccine
Bruna Lo Sasso[SUP] 1 2 [/SUP], Rosaria Vincenza Giglio[SUP] 1 [/SUP], Matteo Vidali[SUP] 3 [/SUP], Concetta Scazzone[SUP] 1 [/SUP], Giulia Bivona[SUP] 1 [/SUP], Caterina Maria Gambino[SUP] 1 2 [/SUP], Anna Maria Ciaccio[SUP] 4 [/SUP], Luisa Agnello[SUP] 1 [/SUP], Marcello Ciaccio[SUP] 1 2 [/SUP]
Affiliations
- PMID: 34206567
- DOI: 10.3390/diagnostics11071135
Abstract
(1) Background: The evaluation of anti-spike protein receptor-binding domain (S-RBD) antibodies represents a useful tool to estimate the individual protection against Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infection; (2) Methods: We evaluated anti S-RBD IgG levels by indirect chemiluminescence immunoassay on Maglumi 800 (SNIBE, California) in 2248 vaccinated subjects without previous SARS-CoV-2 infection, 91 vaccinated individuals recovered from COVID-19, and 268 individuals recovered from COVID-19 who had not been vaccinated. Among those who were healthy and vaccinated, 352 subjects performed a re-dosing after about 72 days from the first measurement. (3) Results: Anti S-RBD IgG levels were lower in subjects with previous infection than vaccinated subjects, with or without previous infection (p < 0.001). No difference was observed between vaccinated subjects, with and without previous SARS-CoV-2 infection. Overall, anti-RBD IgG levels were higher in females than males (2110 vs. 1341 BAU/mL; p < 0.001) as well as in subjects with symptoms after vaccination than asymptomatic ones (2085 vs. 1332 BAU/mL; p = 0.001) and lower in older than younger subjects. Finally, a significant decrease in anti-RBD IgG levels was observed within a short period from a complete two-dose cycle vaccination. (4) Conclusions: Our results show an efficacy antibody response after vaccination with age-, time- and sex-related differences.
Keywords: COVID-19; S-RBD IgG; SARS-CoV-2; antibodies; immunosurvelliance; kinetic; spike; vaccination; vaccine.