tetano
Editor, Senior Moderator
PLoS One. 2019 May 7;14(5):e0216308. doi: 10.1371/journal.pone.0216308. eCollection 2019.
[h=1]Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season.[/h] Maignan M[SUP]1[/SUP], Viglino D[SUP]1[/SUP], Hablot M[SUP]1[/SUP], Termoz Masson N[SUP]1[/SUP], Lebeugle A[SUP]1[/SUP], Collomb Muret R[SUP]1[/SUP], Mabiala Makele P[SUP]1[/SUP], Guglielmetti V[SUP]1[/SUP], Morand P[SUP]2[/SUP], Lupo J[SUP]2[/SUP], Forget V[SUP]3[/SUP], Landelle C[SUP]3[/SUP], Larrat S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]STUDY OBJECTIVE:[/h] To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission.
[h=4]METHODS:[/h] This single-center prospective study recruited adult patients attending the emergency department for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran rapid RT-PCR assays using a dedicated device (cobas Liat, Roche Diagnostics, Meylan, France) located at triage. The same swab sample was also analyzed in the department of virology using conventional RT-PCR techniques. Patients were included 24 hours-a-day, 7 days-a-week. The primary outcome was the diagnostic accuracy of the rapid RT-PCR assay performed at triage.
[h=4]RESULTS:[/h] A total of 187 patients were included over 11 days in January 2018. Median age was 70 years (interquartile range 44 to 84) and 95 (51%) were male. Nine (5%) assays had to be repeated due to failure of the first assay. The sensitivity of the rapid RT-PCR assay performed at triage was 0.98 (95% confidence interval (CI): 0.91-1.00) and the specificity was 0.99 (95% CI: 0.94-1.00). A total of 92 (49%) assays were performed at night-time or during the weekend. The median time from patient entry to rapid RT-PCR assay results was 46 [interquartile range 36-55] minutes.
[h=4]CONCLUSION:[/h] Rapid RT-PCR assay performed by nurses at triage to detect influenza A/B is feasible and highly accurate.
PMID: 31063477 PMCID: PMC6504036 DOI: 10.1371/journal.pone.0216308
Free PMC Article
[h=1]Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season.[/h] Maignan M[SUP]1[/SUP], Viglino D[SUP]1[/SUP], Hablot M[SUP]1[/SUP], Termoz Masson N[SUP]1[/SUP], Lebeugle A[SUP]1[/SUP], Collomb Muret R[SUP]1[/SUP], Mabiala Makele P[SUP]1[/SUP], Guglielmetti V[SUP]1[/SUP], Morand P[SUP]2[/SUP], Lupo J[SUP]2[/SUP], Forget V[SUP]3[/SUP], Landelle C[SUP]3[/SUP], Larrat S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]STUDY OBJECTIVE:[/h] To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission.
[h=4]METHODS:[/h] This single-center prospective study recruited adult patients attending the emergency department for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran rapid RT-PCR assays using a dedicated device (cobas Liat, Roche Diagnostics, Meylan, France) located at triage. The same swab sample was also analyzed in the department of virology using conventional RT-PCR techniques. Patients were included 24 hours-a-day, 7 days-a-week. The primary outcome was the diagnostic accuracy of the rapid RT-PCR assay performed at triage.
[h=4]RESULTS:[/h] A total of 187 patients were included over 11 days in January 2018. Median age was 70 years (interquartile range 44 to 84) and 95 (51%) were male. Nine (5%) assays had to be repeated due to failure of the first assay. The sensitivity of the rapid RT-PCR assay performed at triage was 0.98 (95% confidence interval (CI): 0.91-1.00) and the specificity was 0.99 (95% CI: 0.94-1.00). A total of 92 (49%) assays were performed at night-time or during the weekend. The median time from patient entry to rapid RT-PCR assay results was 46 [interquartile range 36-55] minutes.
[h=4]CONCLUSION:[/h] Rapid RT-PCR assay performed by nurses at triage to detect influenza A/B is feasible and highly accurate.
PMID: 31063477 PMCID: PMC6504036 DOI: 10.1371/journal.pone.0216308
Free PMC Article