tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis
. 2021 Mar 4;100(3):115366.
doi: 10.1016/j.diagmicrobio.2021.115366. Online ahead of print.
Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold value
Aur?lie Schnuriger[SUP] 1 [/SUP], Marine Perrier[SUP] 2 [/SUP], Val?rie Marinho[SUP] 2 [/SUP], Yanne Michel[SUP] 2 [/SUP], Kenda Saloum[SUP] 2 [/SUP], Narjis Boukli[SUP] 2 [/SUP], Sidonie Lambert-Niclot[SUP] 3 [/SUP], Corinne Amiel[SUP] 2 [/SUP], Djeneba Bocar Fofana[SUP] 2 [/SUP], Jo?l Gozlan[SUP] 4 [/SUP], Laurence Morand-Joubert[SUP] 3 [/SUP]
Affiliations
Abstract
RT-PCR is the reference method for diagnosis of a Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection. During the setting up of 6 SARS-CoV-2 RT-PCR assays in our laboratory, comparative evaluations were systematically undertaken and allowed to evidence major discrepancies on cycle threshold RT-PCR results between techniques. These tendencies were confirmed in routine application when analyzing sequential samples from the same patients. Our aim was to examine the impact of the technique among factors influencing RT-PCR result, a far surrogate of 'viral load' in the heterogeneous environment of respiratory specimens.
Copyright ? 2021 Elsevier Inc. All rights reserved.
Conflict of interest statement
Conflict of interest The authors declare no conflict of interest.
. 2021 Mar 4;100(3):115366.
doi: 10.1016/j.diagmicrobio.2021.115366. Online ahead of print.
Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold value
Aur?lie Schnuriger[SUP] 1 [/SUP], Marine Perrier[SUP] 2 [/SUP], Val?rie Marinho[SUP] 2 [/SUP], Yanne Michel[SUP] 2 [/SUP], Kenda Saloum[SUP] 2 [/SUP], Narjis Boukli[SUP] 2 [/SUP], Sidonie Lambert-Niclot[SUP] 3 [/SUP], Corinne Amiel[SUP] 2 [/SUP], Djeneba Bocar Fofana[SUP] 2 [/SUP], Jo?l Gozlan[SUP] 4 [/SUP], Laurence Morand-Joubert[SUP] 3 [/SUP]
Affiliations
- PMID: 33756311
- PMCID: PMC7929791
- DOI: 10.1016/j.diagmicrobio.2021.115366
Abstract
RT-PCR is the reference method for diagnosis of a Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection. During the setting up of 6 SARS-CoV-2 RT-PCR assays in our laboratory, comparative evaluations were systematically undertaken and allowed to evidence major discrepancies on cycle threshold RT-PCR results between techniques. These tendencies were confirmed in routine application when analyzing sequential samples from the same patients. Our aim was to examine the impact of the technique among factors influencing RT-PCR result, a far surrogate of 'viral load' in the heterogeneous environment of respiratory specimens.
Copyright ? 2021 Elsevier Inc. All rights reserved.
Conflict of interest statement
Conflict of interest The authors declare no conflict of interest.