tetano
Editor, Senior Moderator
PLoS One. 2014 Mar 19;9(3):e92306. doi: 10.1371/journal.pone.0092306. eCollection 2014.
Development of Rapid Immunochromatographic Test for Hemagglutinin Antigen of H7 Subtype in Patients Infected with Novel Avian Influenza A (H7N9) Virus.
Kang K1, Chen L2, Zhao X3, Qin C4, Zhan Z2, Wang J2, Li W2, Dzakah EE5, Huang W3, Shu Y3, Jiang T4, Cao W4, Xie M5, Luo X5, Tang S2.
Author information
Abstract
BACKGROUND:
Since human infection with the novel H7N9 avian influenza virus was identified in China in March 2013, the relatively high mortality rate and possibility of human-to-human transmission have highlighted the urgent need for sensitive and specific assays for diagnosis of H7N9 infection.
METHODOLOGY/PRINCIPAL FINDINGS:
We developed a rapid diagnostic test for the novel avian influenza A (H7N9) virus using anti-hemagglutinin (HA) monoclonal antibodies specifically targeting H7 in an immunochromatographic assay system. The assay limit of detection was 103.5 pfu/ml or 103TCID50 of H7N9 virus. The assay specifically detected H7N9 viral isolates and recombinant HA proteins of H7 subtypes including H7N7 and H7N9, but did not react with non-H7 subtypes including H1N1, H3N2, H5N1, H5N9, and H9N2. The detection sensitivity was 59.4% (19/32) for H7N9 patients confirmed by RT-PCR. Moreover, the highest sensitivity of 61.5% (16/26) was obtained when testing H7N9 positive sputum samples while 35.7% (5/14) of nasopharyngeal swabs and 20% (2/10) of fecal samples tested positive. No false positive detection was found when testing 180 H7N9 negative samples.
CONCLUSIONS/SIGNIFICANCE:
Our novel rapid assay can specifically detect H7 HA antigen, facilitating rapid diagnosis for prevention and control of the on-going H7N9 epidemic.
PMID:
24647358
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24647358
Development of Rapid Immunochromatographic Test for Hemagglutinin Antigen of H7 Subtype in Patients Infected with Novel Avian Influenza A (H7N9) Virus.
Kang K1, Chen L2, Zhao X3, Qin C4, Zhan Z2, Wang J2, Li W2, Dzakah EE5, Huang W3, Shu Y3, Jiang T4, Cao W4, Xie M5, Luo X5, Tang S2.
Author information
Abstract
BACKGROUND:
Since human infection with the novel H7N9 avian influenza virus was identified in China in March 2013, the relatively high mortality rate and possibility of human-to-human transmission have highlighted the urgent need for sensitive and specific assays for diagnosis of H7N9 infection.
METHODOLOGY/PRINCIPAL FINDINGS:
We developed a rapid diagnostic test for the novel avian influenza A (H7N9) virus using anti-hemagglutinin (HA) monoclonal antibodies specifically targeting H7 in an immunochromatographic assay system. The assay limit of detection was 103.5 pfu/ml or 103TCID50 of H7N9 virus. The assay specifically detected H7N9 viral isolates and recombinant HA proteins of H7 subtypes including H7N7 and H7N9, but did not react with non-H7 subtypes including H1N1, H3N2, H5N1, H5N9, and H9N2. The detection sensitivity was 59.4% (19/32) for H7N9 patients confirmed by RT-PCR. Moreover, the highest sensitivity of 61.5% (16/26) was obtained when testing H7N9 positive sputum samples while 35.7% (5/14) of nasopharyngeal swabs and 20% (2/10) of fecal samples tested positive. No false positive detection was found when testing 180 H7N9 negative samples.
CONCLUSIONS/SIGNIFICANCE:
Our novel rapid assay can specifically detect H7 HA antigen, facilitating rapid diagnosis for prevention and control of the on-going H7N9 epidemic.
PMID:
24647358
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24647358