tetano
Editor, Senior Moderator
Theranostics. 2018 Nov 29;8(22):6132-6148. doi: 10.7150/thno.28027. eCollection 2018.
[h=1]Development of a smartphone-based rapid dual fluorescent diagnostic system for the simultaneous detection of influenza A and H5 subtype in avian influenza A-infected patients.[/h] Yeo SJ[SUP]1[/SUP], Kang H[SUP]2[/SUP], Dao TD[SUP]1[/SUP], Cuc BT[SUP]1[/SUP], Nguyen ATV[SUP]1[/SUP], Tien TTT[SUP]1[/SUP], Hang NLK[SUP]3[/SUP], Phuong HVM[SUP]3[/SUP], Thanh LT[SUP]3[/SUP], Mai LQ[SUP]3[/SUP], Rah Y[SUP]4[/SUP], Yu K[SUP]4[/SUP], Shin HJ[SUP]5[/SUP], Chong CK[SUP]6[/SUP], Choi HS[SUP]2[/SUP], Park H[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Accurate and rapid diagnosis of highly pathogenic avian influenza A H5N1 is of critical importance for the effective clinical management of patients. Here, we developed a rapid and simultaneous detection toolkit for influenza A H5 subtype viruses in human samples based on a bioconjugate of quantum dots (QDs) assembly and a smartphone-based rapid dual fluorescent diagnostic system (SRDFDS). Methods: Two types of QDs were assembled on a latex bead to enhance the detection sensitivity and specificity of influenza A infection (QD580) and H5 subtype (QD650). The dual signals of influenza A and H5 subtype of H5N1-infected patients were detected simultaneously and quantified separately by SRDFDS equipped with two emission filters. Results: Our results showed a high sensitivity of 92.86% (13/14) and 78.57% (11/14), and a specificity of 100% (38/38, P < 0.0001) and 97.37% (37/38) for influenza A and H5 subtype detection, respectively. Conclusion: Therefore, our multiplex QD bioconjugates and SRDFDS-based influenza virus detection toolkit potentially provide accurate and meaningful diagnosis information with improved detection accuracies and sensitivities for H5N1 patients.
[h=4]KEYWORDS:[/h] H5N1; clinical patients; quantum dot; smartphone-based rapid dual fluorescent diagnostic system
PMID: 30613288 PMCID: PMC6299699 DOI: 10.7150/thno.28027
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[h=1]Development of a smartphone-based rapid dual fluorescent diagnostic system for the simultaneous detection of influenza A and H5 subtype in avian influenza A-infected patients.[/h] Yeo SJ[SUP]1[/SUP], Kang H[SUP]2[/SUP], Dao TD[SUP]1[/SUP], Cuc BT[SUP]1[/SUP], Nguyen ATV[SUP]1[/SUP], Tien TTT[SUP]1[/SUP], Hang NLK[SUP]3[/SUP], Phuong HVM[SUP]3[/SUP], Thanh LT[SUP]3[/SUP], Mai LQ[SUP]3[/SUP], Rah Y[SUP]4[/SUP], Yu K[SUP]4[/SUP], Shin HJ[SUP]5[/SUP], Chong CK[SUP]6[/SUP], Choi HS[SUP]2[/SUP], Park H[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Accurate and rapid diagnosis of highly pathogenic avian influenza A H5N1 is of critical importance for the effective clinical management of patients. Here, we developed a rapid and simultaneous detection toolkit for influenza A H5 subtype viruses in human samples based on a bioconjugate of quantum dots (QDs) assembly and a smartphone-based rapid dual fluorescent diagnostic system (SRDFDS). Methods: Two types of QDs were assembled on a latex bead to enhance the detection sensitivity and specificity of influenza A infection (QD580) and H5 subtype (QD650). The dual signals of influenza A and H5 subtype of H5N1-infected patients were detected simultaneously and quantified separately by SRDFDS equipped with two emission filters. Results: Our results showed a high sensitivity of 92.86% (13/14) and 78.57% (11/14), and a specificity of 100% (38/38, P < 0.0001) and 97.37% (37/38) for influenza A and H5 subtype detection, respectively. Conclusion: Therefore, our multiplex QD bioconjugates and SRDFDS-based influenza virus detection toolkit potentially provide accurate and meaningful diagnosis information with improved detection accuracies and sensitivities for H5N1 patients.
[h=4]KEYWORDS:[/h] H5N1; clinical patients; quantum dot; smartphone-based rapid dual fluorescent diagnostic system
PMID: 30613288 PMCID: PMC6299699 DOI: 10.7150/thno.28027
Free full text