tetano
Editor, Senior Moderator
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Sci Rep. 2017 Feb 20;7:42963. doi: 10.1038/srep42963.
[h=1]Detection of viral respiratory pathogens in mild and severe acute respiratory infections in Singapore.[/h] Jiang L[SUP]1[/SUP], Lee VJ[SUP]1,[/SUP][SUP]2[/SUP], Cui L[SUP]3[/SUP], Lin R[SUP]3,[/SUP][SUP]4[/SUP], Tan CL[SUP]5[/SUP], Tan LW[SUP]1[/SUP], Lim WY[SUP]1[/SUP], Leo YS[SUP]5[/SUP], Low L[SUP]6[/SUP], Hibberd M[SUP]6[/SUP], Chen MI[SUP]1,[/SUP][SUP]7[/SUP].
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[h=3]Abstract[/h] To investigate the performance of laboratory methods and clinical case definitions in detecting the viral pathogens for acute respiratory infections (ARIs) from a prospective community cohort and hospital inpatients, nasopharyngeal swabs from cohort members reporting ARIs (community-ARI) and inpatients admitted with ARIs (inpatient-ARI) were tested by Singleplex Real Time-Polymerase Chain Reaction (SRT-PCR), multiplex RT-PCR (MRT-PCR) and pathogen-chip system (PathChip) between April 2012 and December 2013. Community-ARI and inpatient-ARI was also combined with mild and severe cases of influenza from a historical prospective study as mild-ARI and severe-ARI respectively to evaluate the performance of clinical case definitions. We analysed 130 community-ARI and 140 inpatient-ARI episodes (5 inpatient-ARI excluded because multiple pathogens were detected), involving 138 and 207 samples respectively. Detection by PCR declined with days post-onset for influenza virus; decrease was faster for community-ARI than for inpatient-ARI. No such patterns were observed for non-influenza respiratory virus infections. PathChip added substantially to viruses detected for community-ARI only. Clinical case definitions discriminated influenza from other mild-ARI but performed poorly for severe-ARI and for older participants. Rational strategies for diagnosis and surveillance of influenza and other respiratory virus must acknowledge the differences between ARIs presenting in community and hospital settings.
PMID: 28218288 DOI: 10.1038/srep42963
[PubMed - in process]
Sci Rep. 2017 Feb 20;7:42963. doi: 10.1038/srep42963.
[h=1]Detection of viral respiratory pathogens in mild and severe acute respiratory infections in Singapore.[/h] Jiang L[SUP]1[/SUP], Lee VJ[SUP]1,[/SUP][SUP]2[/SUP], Cui L[SUP]3[/SUP], Lin R[SUP]3,[/SUP][SUP]4[/SUP], Tan CL[SUP]5[/SUP], Tan LW[SUP]1[/SUP], Lim WY[SUP]1[/SUP], Leo YS[SUP]5[/SUP], Low L[SUP]6[/SUP], Hibberd M[SUP]6[/SUP], Chen MI[SUP]1,[/SUP][SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] To investigate the performance of laboratory methods and clinical case definitions in detecting the viral pathogens for acute respiratory infections (ARIs) from a prospective community cohort and hospital inpatients, nasopharyngeal swabs from cohort members reporting ARIs (community-ARI) and inpatients admitted with ARIs (inpatient-ARI) were tested by Singleplex Real Time-Polymerase Chain Reaction (SRT-PCR), multiplex RT-PCR (MRT-PCR) and pathogen-chip system (PathChip) between April 2012 and December 2013. Community-ARI and inpatient-ARI was also combined with mild and severe cases of influenza from a historical prospective study as mild-ARI and severe-ARI respectively to evaluate the performance of clinical case definitions. We analysed 130 community-ARI and 140 inpatient-ARI episodes (5 inpatient-ARI excluded because multiple pathogens were detected), involving 138 and 207 samples respectively. Detection by PCR declined with days post-onset for influenza virus; decrease was faster for community-ARI than for inpatient-ARI. No such patterns were observed for non-influenza respiratory virus infections. PathChip added substantially to viruses detected for community-ARI only. Clinical case definitions discriminated influenza from other mild-ARI but performed poorly for severe-ARI and for older participants. Rational strategies for diagnosis and surveillance of influenza and other respiratory virus must acknowledge the differences between ARIs presenting in community and hospital settings.
PMID: 28218288 DOI: 10.1038/srep42963
[PubMed - in process]