tetano
Editor, Senior Moderator
Adv Virol. 2014;2014:274679. doi: 10.1155/2014/274679. Epub 2014 Dec 9.
[h=1]Detection of influenza virus infection using two PCR methods.[/h] Zimmerman RK[SUP]1[/SUP], Rinaldo CR[SUP]2[/SUP], Nowalk MP[SUP]1[/SUP], Balasubramani GK[SUP]3[/SUP], Thompson MG[SUP]4[/SUP], Bullotta A[SUP]5[/SUP], Susick M[SUP]1[/SUP], Wisniewski S[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses.
PMID: 25574169 [PubMed]
http://www.ncbi.nlm.nih.gov/pubmed/25574169
[h=1]Detection of influenza virus infection using two PCR methods.[/h] Zimmerman RK[SUP]1[/SUP], Rinaldo CR[SUP]2[/SUP], Nowalk MP[SUP]1[/SUP], Balasubramani GK[SUP]3[/SUP], Thompson MG[SUP]4[/SUP], Bullotta A[SUP]5[/SUP], Susick M[SUP]1[/SUP], Wisniewski S[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses.
PMID: 25574169 [PubMed]
http://www.ncbi.nlm.nih.gov/pubmed/25574169