tetano
Editor, Senior Moderator
Appl Opt. 2019 Apr 10;58(11):2839-2844. doi: 10.1364/AO.58.002839.
[h=1]Detection of influenza virus by electrochemical surface plasmon resonance under potential modulation.[/h] Qatamin AH, Ghithan JH, Moreno M, Nunn BM, Jones KB, Zamborini FP, Keynton RS, O'Toole MG, Mendes SB.
[h=3]Abstract[/h] In this study we report the development of a novel viral pathogen immunosensor technology based on the electrochemical modulation of the optical signal from a surface plasmon wave interacting with a redox dye reporter. The device is formed by incorporating a sandwich immunoassay onto the surface of a plasmonic device mounted in a micro-electrochemical flow cell, where it is functionalized with a monoclonal antibody aimed to a specific target pathogen antigen. Once the target antigen is bound to the surface, it promotes the capturing of a secondary polyclonal antibody that has been conjugated with a redox-active methylene blue dye. The methylene blue displays a reversible change in the complex refractive index throughout a reduction-oxidation transition, which generates an optical signal that can be electrochemically modulated and detected at high sensitivity. For proof-of-principle measurements, we have targeted the hemagglutinin protein from the H5N1 avian influenza A virus to demonstrate the capabilities of our device for detection and quantification of a critical influenza antigen. Our experimental results of the EC-SPR-based immunosensor under potential modulation showed a 300 pM limit of detection for the H5N1 antigen.
PMID: 31044886 DOI: 10.1364/AO.58.002839
[h=1]Detection of influenza virus by electrochemical surface plasmon resonance under potential modulation.[/h] Qatamin AH, Ghithan JH, Moreno M, Nunn BM, Jones KB, Zamborini FP, Keynton RS, O'Toole MG, Mendes SB.
[h=3]Abstract[/h] In this study we report the development of a novel viral pathogen immunosensor technology based on the electrochemical modulation of the optical signal from a surface plasmon wave interacting with a redox dye reporter. The device is formed by incorporating a sandwich immunoassay onto the surface of a plasmonic device mounted in a micro-electrochemical flow cell, where it is functionalized with a monoclonal antibody aimed to a specific target pathogen antigen. Once the target antigen is bound to the surface, it promotes the capturing of a secondary polyclonal antibody that has been conjugated with a redox-active methylene blue dye. The methylene blue displays a reversible change in the complex refractive index throughout a reduction-oxidation transition, which generates an optical signal that can be electrochemically modulated and detected at high sensitivity. For proof-of-principle measurements, we have targeted the hemagglutinin protein from the H5N1 avian influenza A virus to demonstrate the capabilities of our device for detection and quantification of a critical influenza antigen. Our experimental results of the EC-SPR-based immunosensor under potential modulation showed a 300 pM limit of detection for the H5N1 antigen.
PMID: 31044886 DOI: 10.1364/AO.58.002839