tetano
Editor, Senior Moderator
J Virol Methods. 2014 Oct 14;211C:12-18. doi: 10.1016/j.jviromet.2014.10.002. [Epub ahead of print]
Detection of influenza A virus subtypes using a solid-phase PCR microplate chip assay.
Sun XC1, Wang Y2, Yang L3, Zhang H4.
Author information
Abstract
A rapid and sensitive microplate chip based on solid PCR was developed to identify influenza A subtypes. A simple ultraviolet cross-linking method was used to immobilize DNA probes on pretreated microplates. Solid-phase PCR was proven to be a convenient method for influenza A screening. The sensitivity of the microplate chip was 10-3μg/mL for the enzymatic colorimetric method and 10-4μg/mL for the fluorescence method. The 10 sets of primers and probes for the microplate chip were highly specific and did not interfere with each other. These results suggest that the microplate chip based on solid PCR can be used to rapidly detect universal influenza A and its subtypes. This platform can also be used to detect other pathogenic microorganisms.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Influenza A virus; Microplate chip; Solid-phase RT-PCR
PMID:
25447756
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25447756
Detection of influenza A virus subtypes using a solid-phase PCR microplate chip assay.
Sun XC1, Wang Y2, Yang L3, Zhang H4.
Author information
Abstract
A rapid and sensitive microplate chip based on solid PCR was developed to identify influenza A subtypes. A simple ultraviolet cross-linking method was used to immobilize DNA probes on pretreated microplates. Solid-phase PCR was proven to be a convenient method for influenza A screening. The sensitivity of the microplate chip was 10-3μg/mL for the enzymatic colorimetric method and 10-4μg/mL for the fluorescence method. The 10 sets of primers and probes for the microplate chip were highly specific and did not interfere with each other. These results suggest that the microplate chip based on solid PCR can be used to rapidly detect universal influenza A and its subtypes. This platform can also be used to detect other pathogenic microorganisms.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Influenza A virus; Microplate chip; Solid-phase RT-PCR
PMID:
25447756
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25447756