tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2020 Jan 19. doi: 10.1111/irv.12712. [Epub ahead of print] [h=1]Detection of anti-NS1 antibodies after pandemic influenza exposure: Evaluation of a serological method for distinguishing H1N1pdm09 infected from vaccinated cases.[/h]
Robertson AH[SUP]1[/SUP], Mahic M[SUP]1,[/SUP][SUP]2[/SUP], Savic M[SUP]1[/SUP], Tunheim G[SUP]1,[/SUP][SUP]3[/SUP], Hungnes O[SUP]1,[/SUP][SUP]4[/SUP], Trogstad L[SUP]1[/SUP], Lipkin WI[SUP]2[/SUP], Mjaaland S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Reliable exposure information is crucial for assessing health outcomes of influenza infection and vaccination. Current serological methods are unable to distinguish between anti-hemagglutinin (HA) antibodies induced by infection or vaccination.
[h=4]OBJECTIVES:[/h] We aimed to explore an alternative method for differentiating influenza infection and vaccination.
[h=4]METHODS:[/h] Sera from animals inoculated with influenza viruses or purified H1N1pdm09 HA were obtained. Human samples were selected from a pregnancy cohort established during the 2009 H1N1 pandemic. Unvaccinated, laboratory-confirmed cases (N = 18), vaccinated cases without influenza-like-illness (N = 18) and uninfected, unvaccinated controls (N = 18) were identified based on exposure data from questionnaires, national registries and maternal hemagglutination inhibition (HI) titres at delivery. Animal and human samples were tested for antibodies against the non-structural protein 1 (NS1) and HA from H1N1pdm09, using a Luciferase Immunoprecipitation System (LIPS).
[h=4]RESULTS:[/h] Anti-NS1 H1N1pdm09 antibodies were detected in sera from experimentally infected, but not from vaccinated, animals. Anti-HA H1N1pdm09 antibodies were detectable after either of these exposures. In human samples, 28% of individuals with laboratory-confirmed influenza were seropositive for H1N1pdm09 NS1, whereas vaccinated cases and controls were seronegative. There was a trend for H1N1pdm09 NS1 seropositive cases reporting more severe and longer duration of symptomatic illness than seronegative cases. Anti-HA H1N1pdm09 antibodies were detected in all cases and in 61% of controls.
[h=4]CONCLUSIONS:[/h] The LIPS method could differentiate between sera from experimentally infected and vaccinated animals. However, in human samples obtained more than 6 months after the pandemic, LIPS was specific, but not sufficiently sensitive for ascertaining cases by exposure.
? 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] H1N1pdm09; NS1; influenza; pandemic; serology; vaccination
PMID: 31955522 DOI: 10.1111/irv.12712
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Robertson AH[SUP]1[/SUP], Mahic M[SUP]1,[/SUP][SUP]2[/SUP], Savic M[SUP]1[/SUP], Tunheim G[SUP]1,[/SUP][SUP]3[/SUP], Hungnes O[SUP]1,[/SUP][SUP]4[/SUP], Trogstad L[SUP]1[/SUP], Lipkin WI[SUP]2[/SUP], Mjaaland S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Reliable exposure information is crucial for assessing health outcomes of influenza infection and vaccination. Current serological methods are unable to distinguish between anti-hemagglutinin (HA) antibodies induced by infection or vaccination.
[h=4]OBJECTIVES:[/h] We aimed to explore an alternative method for differentiating influenza infection and vaccination.
[h=4]METHODS:[/h] Sera from animals inoculated with influenza viruses or purified H1N1pdm09 HA were obtained. Human samples were selected from a pregnancy cohort established during the 2009 H1N1 pandemic. Unvaccinated, laboratory-confirmed cases (N = 18), vaccinated cases without influenza-like-illness (N = 18) and uninfected, unvaccinated controls (N = 18) were identified based on exposure data from questionnaires, national registries and maternal hemagglutination inhibition (HI) titres at delivery. Animal and human samples were tested for antibodies against the non-structural protein 1 (NS1) and HA from H1N1pdm09, using a Luciferase Immunoprecipitation System (LIPS).
[h=4]RESULTS:[/h] Anti-NS1 H1N1pdm09 antibodies were detected in sera from experimentally infected, but not from vaccinated, animals. Anti-HA H1N1pdm09 antibodies were detectable after either of these exposures. In human samples, 28% of individuals with laboratory-confirmed influenza were seropositive for H1N1pdm09 NS1, whereas vaccinated cases and controls were seronegative. There was a trend for H1N1pdm09 NS1 seropositive cases reporting more severe and longer duration of symptomatic illness than seronegative cases. Anti-HA H1N1pdm09 antibodies were detected in all cases and in 61% of controls.
[h=4]CONCLUSIONS:[/h] The LIPS method could differentiate between sera from experimentally infected and vaccinated animals. However, in human samples obtained more than 6 months after the pandemic, LIPS was specific, but not sufficiently sensitive for ascertaining cases by exposure.
? 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] H1N1pdm09; NS1; influenza; pandemic; serology; vaccination
PMID: 31955522 DOI: 10.1111/irv.12712
Free full text