tetano
Editor, Senior Moderator
Virology. 2018 Sep 10;524:182-191. doi: 10.1016/j.virol.2018.08.021. [Epub ahead of print]
[h=1]Design and validation of a universal influenza virus enrichment probe set and its utility in deep sequence analysis of primary cloacal swab surveillance samples of wild birds.[/h] Xiao Y[SUP]1[/SUP], Nolting JM[SUP]2[/SUP], Sheng ZM[SUP]3[/SUP], Bristol T[SUP]3[/SUP], Qi L[SUP]3[/SUP], Bowman AS[SUP]2[/SUP], Taubenberger JK[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus infections in humans and animals are major public health concerns. In the current study, a set of universal influenza enrichment probes was developed to increase the sensitivity of sequence-based virus detection and characterization for all influenza viruses. This universal influenza enrichment probe set contains 46,953 120nt RNA biotin-labeled probes designed based on all available influenza viral sequences and it can be used to enrich for influenza sequences without prior knowledge of type or subtype. Marked enrichment was demonstrated in influenza A/H1N1, influenza B, and H1-to-H16 hemagglutinin plasmids spiked into human DNA and in cultured influenza A/H2N1 virus. Furthermore, enrichment effects and mixed influenza A virus infections were revealed in wild bird cloacal swab samples. Therefore, this universal influenza virus enrichment probe system can capture and enrich influenza viral sequences selectively and effectively in different samples, especially ones with degraded RNA or containing low amount of influenza RNA.
[h=4]KEYWORDS:[/h] Deep sequencing; Influenza; Mixed avian influenza infection; Universal influenza enrichment
PMID: 30212665 DOI: 10.1016/j.virol.2018.08.021
[h=1]Design and validation of a universal influenza virus enrichment probe set and its utility in deep sequence analysis of primary cloacal swab surveillance samples of wild birds.[/h] Xiao Y[SUP]1[/SUP], Nolting JM[SUP]2[/SUP], Sheng ZM[SUP]3[/SUP], Bristol T[SUP]3[/SUP], Qi L[SUP]3[/SUP], Bowman AS[SUP]2[/SUP], Taubenberger JK[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza virus infections in humans and animals are major public health concerns. In the current study, a set of universal influenza enrichment probes was developed to increase the sensitivity of sequence-based virus detection and characterization for all influenza viruses. This universal influenza enrichment probe set contains 46,953 120nt RNA biotin-labeled probes designed based on all available influenza viral sequences and it can be used to enrich for influenza sequences without prior knowledge of type or subtype. Marked enrichment was demonstrated in influenza A/H1N1, influenza B, and H1-to-H16 hemagglutinin plasmids spiked into human DNA and in cultured influenza A/H2N1 virus. Furthermore, enrichment effects and mixed influenza A virus infections were revealed in wild bird cloacal swab samples. Therefore, this universal influenza virus enrichment probe system can capture and enrich influenza viral sequences selectively and effectively in different samples, especially ones with degraded RNA or containing low amount of influenza RNA.
[h=4]KEYWORDS:[/h] Deep sequencing; Influenza; Mixed avian influenza infection; Universal influenza enrichment
PMID: 30212665 DOI: 10.1016/j.virol.2018.08.021