tetano
Editor, Senior Moderator
Cytokine
. 2021 Jan 28;141:155455.
doi: 10.1016/j.cyto.2021.155455. Online ahead of print.
Prognostic bioindicators in severe COVID-19 patients
L Bergantini[SUP] 1 [/SUP], E Bargagli[SUP] 2 [/SUP], M d'Alessandro[SUP] 2 [/SUP], R M Refini[SUP] 2 [/SUP], P Cameli[SUP] 2 [/SUP], L Galasso[SUP] 3 [/SUP], C Scapellato[SUP] 3 [/SUP], F Montagnani[SUP] 4 [/SUP], S Scolletta[SUP] 5 [/SUP], F Franchi[SUP] 5 [/SUP], S Valente[SUP] 6 [/SUP], D Bennett[SUP] 2 [/SUP], G Sebastiani[SUP] 7 [/SUP], B Frediani[SUP] 8 [/SUP], F Dotta[SUP] 7 [/SUP]
Affiliations
Abstract
Background: Severe acute respiratory syndrome caused by novel coronavirus 2 (SARS-CoV-2) emerged in Wuhan (China) in December 2019. Here we evaluated a panel of biomarkers to phenotype patients and to define the role of immuno-inflammatory mediators as biomarkers of severity.
Materials and methods: Serum samples were obtained from 24 COVID-19 patients on admission to hospital, before any treatment or infusion of intravenous steroids or invasive ventilation. KL-6 IL-6 and C-peptide were measured by chemiluminescent enzyme immunoassay. IL-6 assay was validated for accuracy and precision. The validity of variables used to distinguish severe from mild-to-moderate patients was assessed by areas under curves (AUC) of the receiver operating characteristic (ROC) and logistic regression was performed to combine parameters of the two groups.
Results: In the severe group, IL-6, CRP and KL-6 concentrations were significantly higher than in mild-to-moderate patients. KL-6, IL-6 and CRP concentrations were directly correlated with each other. ROC curve analysis of the logistic regression model including IL-6, KL-6 and CRP showed the best performance with an AUC of 0.95.
Conclusions: Besides corroborating previous reports of over-expression of IL-6 in severe COVID-19 patients requiring mechanical ventilation, analytical determination of other mediators showed that IL-6 concentrations were correlated with those of KL-6 and CRP. The combination of these three prognostic bioindicators made it possible to distinguish severe COVID-19 patients with poor prognosis from mild-to-moderate patients.
Keywords: Biomarkers; COVID-19; IL-6; KL-6.
. 2021 Jan 28;141:155455.
doi: 10.1016/j.cyto.2021.155455. Online ahead of print.
Prognostic bioindicators in severe COVID-19 patients
L Bergantini[SUP] 1 [/SUP], E Bargagli[SUP] 2 [/SUP], M d'Alessandro[SUP] 2 [/SUP], R M Refini[SUP] 2 [/SUP], P Cameli[SUP] 2 [/SUP], L Galasso[SUP] 3 [/SUP], C Scapellato[SUP] 3 [/SUP], F Montagnani[SUP] 4 [/SUP], S Scolletta[SUP] 5 [/SUP], F Franchi[SUP] 5 [/SUP], S Valente[SUP] 6 [/SUP], D Bennett[SUP] 2 [/SUP], G Sebastiani[SUP] 7 [/SUP], B Frediani[SUP] 8 [/SUP], F Dotta[SUP] 7 [/SUP]
Affiliations
- PMID: 33548798
- DOI: 10.1016/j.cyto.2021.155455
Abstract
Background: Severe acute respiratory syndrome caused by novel coronavirus 2 (SARS-CoV-2) emerged in Wuhan (China) in December 2019. Here we evaluated a panel of biomarkers to phenotype patients and to define the role of immuno-inflammatory mediators as biomarkers of severity.
Materials and methods: Serum samples were obtained from 24 COVID-19 patients on admission to hospital, before any treatment or infusion of intravenous steroids or invasive ventilation. KL-6 IL-6 and C-peptide were measured by chemiluminescent enzyme immunoassay. IL-6 assay was validated for accuracy and precision. The validity of variables used to distinguish severe from mild-to-moderate patients was assessed by areas under curves (AUC) of the receiver operating characteristic (ROC) and logistic regression was performed to combine parameters of the two groups.
Results: In the severe group, IL-6, CRP and KL-6 concentrations were significantly higher than in mild-to-moderate patients. KL-6, IL-6 and CRP concentrations were directly correlated with each other. ROC curve analysis of the logistic regression model including IL-6, KL-6 and CRP showed the best performance with an AUC of 0.95.
Conclusions: Besides corroborating previous reports of over-expression of IL-6 in severe COVID-19 patients requiring mechanical ventilation, analytical determination of other mediators showed that IL-6 concentrations were correlated with those of KL-6 and CRP. The combination of these three prognostic bioindicators made it possible to distinguish severe COVID-19 patients with poor prognosis from mild-to-moderate patients.
Keywords: Biomarkers; COVID-19; IL-6; KL-6.